This kit has been developed as a reagent for research purposes to detect respective subtypic determinants, d, y, r, and w, in hepatitis B surface antigen (HBsAg) positive samples for identification of HBsAg subtypes such as adr, adw, ayr and ayw.
Detection of subtypic determinants is based on the solid-phase sandwich EIA (Enzyme Immuno Assay). 96 wells of the microplate are coated with monoclonal antibody against the common determinant a of HBsAg. HBsAg in samples dispensed to wells is caught on the solid phase and their subtypic determinant, d, y, r, or w, is detected by peroxidase-labeled monoclonal antibody against corresponding determinant.
☆ Subtypes of HBsAg can be determined by solid-phase sandwich EIA (Enzyme Immuno Assay)
☆ 96-well (8 wells/strip x 12) solid phase microplate coated with anti-HBs a
☆ Total reaction time is 5.5 hours
Determination of subtypes of hepatitis B surface antigen (HBsAg) in serum or plasma
|1||Microplate coated with anti-HBs a (8 wells/strip x 12)||1 plate.|
|2||HBsAg negative control||1 mL x 1 vial|
|3||HBsAg positive control (d) green||0.5 mL x 1 vial|
|4||HBsAg positive control (y) yellow||0.5 mL x 1 vial|
|5||HBsAg positive control (r) red||0.5 mL x 1 vial|
|6||HBsAg positive control (w) white||0.5 mL x 1 vial|
|7||Enzyme labeled monoclonal antibody (d) green||1.5 mL x 1 vial|
|8||Enzyme labeled monoclonal antibody (y) yellow||1.5 mL x 1 vial|
|9||Enzyme labeled monoclonal antibody (r) red.||1.5 mL x 1 vial|
|10||Enzyme labeled monoclonal antibody (w) white||1.5 mL x 1 vial|
|11||Enzyme substrate (3, 3', 5, 5'-tetramethylbenzidine)||10 mL x 1 vial|
|12||Reaction stopper||10 mL x 1 vial|
|13||20x concentrated washing solution (containing detergent)||25 mL x 2 vial|
|14||Plate seal||3 sheets.|
HBsAg Subtype EIA
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.