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Immunoglobulin kappa chain analysis

Rabbit anti Immunoglobulin κ light chain polyclonal antibody

This polyclonal antibody was produced against rat immunoglobulin κ light chain peptide (SVTDQDSKDSTYSMSSTLSL)1,2 . It cross-react with some other species (see immunoblot in figure1). It can be used western blot and dot blot.

Western blot

Figure 1
Western blot of serum of variaus species with the rabbit anti-immunoglobulin κ light chain polyclonal antibody 1: Rat; 2: Mouse; 3:Marmoset. In the species, Only a band at <30k (neighboring 24k ) is detected.



Protocol

Procedure for western blot of sera with rabbit anti-immunoglobulin κ light chain polyclonal antibodies

Materials
  • Rat or mouse sea
  • Extracting buffer solutions (100 mM Tris-HCl, pH 8.8, 2% SDS, 20% sucrose, 0.06% BPB, 7M urea, 2M thiourea and 0.1M DTT)
  • SDS-PAGE gel (10-20% gradient or 12.5% isocratically)
  • PVDF or nitrocellulose membrane
  • Blocking buffer (BSA or skim milk etc)
  • RESVO rabbit anti- immunoglobulin κ light chain polyclonal antibody
  • HRP conjugated anti-rabbit IgG
  • ECL western blot substrate
Methods
  1. Sera mixed at a volume ratio of 1:1 with protein extracting buffer solutions(Vortex more than 15 min).
  2. The mixture was subjected to SDS-PAGE gel .
  3. Electrophoresed proteins were transferred from the SDS-PAGE gel to a PVDF (or nitrocellulose) membrane.
  4. The membrane is blocked for 1 hr at room temperature in a blocking buffer solution (Including BSA or skim milk etc).
  5. The membrane was then incubated overnight at 4°C in a blocking buffer solution containing 1:500-2000 diluted anti-immunoglobulin κ light chain polyclonal antibody and finally transferred into a blocking buffer solution containing a HRP conjugated anti-rabbit IgG.
  6. Chemiluminescence from HRP elicited by ECL western blot substrate was detected by western blot imager.

Notice
Rat or mouse serum samples should diluted from 10-100 times with PBS etc, because rodent serum include high concentration of immunoglobulin κ light chain.

Product List

Product Name Cat# Quantity Price

Anti Immunoglobulin (KAPPA) light chain (RESVO Anti-IgK)

RES-IGKR-P1IGG 100UL

¥ 20,000
$ 267
€ 200

References
  • Oh-Nishi et al. A possible serologic biomarker for maternal immune activation-associated neurodevelopmental disorders found in the rat models. Neurosci Res. 2016 Dec;113:63-70. PMID:27425770

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.