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very convenient tool for evaluating sugar chain - protein interaction

Gold Nano Particles for Bioseparation SGNP (Sugar chain immobilized Gold Nano Particle)

Background

SGNP (Sugar chain - immobilized Gold Nano-Particle) is a gold nano particle immobilized with structurally defined sugar chains, showing purple-red color (Abs. max = ca. 530 nm). In the present product (Heparin-GNP), heparin, a sulfated polysaccharide, is immobilized.The diameter of particle is ca.15 to 30 nm.

Application

[Application#1]
Concentration and Purification of Heparin Binding Sample (Protein, Virus, etc.) from a Crude Extract

Heparin binding sample can be easily concentrated/captured by mixing Heparin-GNP with a crude extract, followed by the centrifugation. The concentrated/captured heparin binding sample with Heparin-GNP can be analyzed by SDS-PAGE, Western-blotting, real-time PCR, etc.

Example for Identification-1: SDS-PAGE and Western-blotting
To the precipitate obtained using the protocol, add 10-30 μl of sample preparation buffer*, and boil for 10 min. The sample is directly analyzed by SDS-PAGE and Western-blot.

Example for Identification-2: Real-time PCR
To the precipitate obtained in the protocol, add 10 μl of H20, and boil for 10 min. The boiled samples are ready to use for Real-time PCR

[Application #2]
Probe for Dot-Blot Assay
The samples ability to bind to heparin can be visualized by soaking the nitrocellulose membrane spotted with sample in a solution of Heparin-GNP.

Product List

Product Name Cat# Quantity Price

(ALPHA)Glucosyl GNP

SXB-G-00M-250-EX 1VIAL

¥ 7,000
$ 94
€ 70

(BETA)Glucosyl GNP

SXB-G-00C-250-EX 1VIAL

¥ 7,000
$ 94
€ 70

(ALPHA)Galactosyl GNP

SXB-G-00E-250-EX 1VIAL

¥ 7,000
$ 94
€ 70

(BETA)Galactosyl GNP

SXB-G-00L-250-EX 1VIAL

¥ 7,000
$ 94
€ 70

(BETA)GlcNAc GNP

SXB-G-BGN-250-EX 1VIAL

¥ 18,000
$ 240
€ 180

(ALPHA)GalNAc GNP

SXB-G-AAN-250-EX 1VIAL

¥ 18,000
$ 240
€ 180

(BETA)GalNAc GNP

SXB-G-BAN-250-EX 1VIAL

¥ 18,000
$ 240
€ 180

(ALPHA)Fucosyl GNP

SXB-G-0AF-250-EX 1VIAL

¥ 18,000
$ 240
€ 180

(BETA)Fucosyl GNP

SXB-G-0BF-250-EX 1VIAL

¥ 18,000
$ 240
€ 180

(ALPHA)Mannosyl GNP

SXB-G-AMA-250-EX 1VIAL

¥ 9,000
$ 120
€ 90

3SialylGalactosyl GNP

SXB-G-03S-250-EX 1VIAL

¥ 18,000
$ 240
€ 180

6SialylGalactosyl GNP

SXB-G-06S-250-EX 1VIAL

¥ 18,000
$ 240
€ 180

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.