This unit was developed for the purpose of simplifying freezing-crushing procedure. You do not need to grind liquid nitrogen in a mortar, which is a very intricate procedure.
As commercially available, disposable closed type plastic sample tube can be used, it is possible for you to prevent samples from scattering and contaminating. With increased working efficiency, a lot of samples can be treated quickly. We would like to ask you to consider for using this unit!
Example1: freezing-crushing of yeast
1.Culture yeast (S. cerevisiae) in YPD medium until exponential growth phase.
2.Collect fungi from 1ml culture in 2ml sample tube using centrifuge.
3.Then, freeze and crush them by SK MILL.
4.Eliminate fungal residue and recover nucleic acid by phenol extraction and ethanol precipitation.
5.Apply it to electrophoresis.
Example2 : RNA extraction from green leaves of rice
1.Cut green leaves of rice ( ca. 1 gram) into small pieces by scissors.
2.Then, freeze and crush them by SK MILL.
3.Extract RNA by CTAB ( cetyltrimethylammonium bromide) method.
4.Apply it to electrophoresis.