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World's First Fluorogenic reagent !

IST Fluolid

Fluolid is


Fluolid flyer download [PDF]

Feature and Advantages

1. high quantum yield in the solid state
2. no photobleach
3. high stability for light, heat and pH
4. labeling rate is higher than that of traditional dye

[Wavelength (Ex and Em) and Stokes shift of IST Fluolid]

UV spectra of Fluolid labeled IgG (left: green, center: yellow, right: orange

[Compared IST Fluolid to Traditional Dye]
  IST Fluolid Traditional Dye
Stability of Light good
(Stable over 2 years under daylight )
bad
Stability of Temperature good
(over 200°C)
bad
(degrade at r.t.)
Stability of pH good
(stable for pH1-14)
bad
(usable pH5-9)
Stokes Shifts Wide
(about 100-150nm)
Narrow
(about 10-50nm)
Fluorescent
in the solid state
Highly Fluorescent quenching
Storage Condition Under room temperature* Under -20°C

* The fluorescence unit of “IST Fluolid” has high stability under various conditions.But, to keep the activity of the succinimidyl ester group, please store at 2-8 °C.

Usage

[Labeling of Oligo DNA with IST Fluolid]


・ Almost 90-100% labeling of P72 oligo DNA (traditional dye : 20-80%).
・ Measurement at low concentration with existing micro imager.
・ Detectable of the dried sample at low concentration (~10-12mol).

[Labeling of BSA using IST Fluolid]

[Labeled GFP antibody]

・The labeling of GFP antibody advanced quantity.
・The labeled GFP antibody keeps the activity.
・The labeled GFP antibody was trapped by fixed EGFP.

Application

[Observation of Multi-color Immunostaining-1]

rotocols of Multi-Color ImmunostainingPDF

[Observation of Multi-color Immunostaining-2]
(Fluolid-W Orange 600 exclusive filter)

[Observation of Lectin staining]

Protocols of Lectin ImmunostainingPDF