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To detect allergic proteins in foods or solutions

FASTKIT SLIM "EGG", "MILK", "WHEAT", "BUCKWHEAT", "PEANUT" and "SOYBEAN"

NPH_NFS001_01.jpg

Background

The Food Sanitation Law obliges that 7 specified allergenic ingredients (egg, milk, wheat, buckwheat, peanut, shrimp and crab), which have a high risk of inducing food allergies, should be listed on food labels. The Food Sanitation Law also recommends that 18 ingredients, including soybeans, should be listed on labels like specified allergenic ingredients.
“Providing accurate information by listing of ingredients on food labels” and “preventing contamination with the ingredients which are not listed on the labels,” are required to prevent food allergies. Moreover, as a measure to prevent contamination, routine management at the manufacturing sites, including verification of the process of washing machines and devices used for production, is important. Tests can be conducted rapidly and simply with this kit.

Feature and Advantages

#  The simple one-step operation enables easy assessment, and anybody can perform the test easily.
#  Because there is no need for special test equipment, and the test gives rapid results (in 15 minutes), it is ideal for routine management at production sites.

1) Sensitivity Test
The results of tests conducted in accordance with instructions for “the preparation of the sample solution” and “the operating procedures” described in this instruction manual will be positive when the concentration of buckwheat protein in the sample solution is 25 ng/mL or more.

2)  Reproducibility Test
When positive and negative sample solutions of buckwheat protein were simultaneously tested three times each, all positive sample solutions exhibited positive results and all negative sample solutions show negative results.

3)  False Positive / False Negative Results
(1) Cross-reactivity with the specified ingredients other than buckwheat (egg, milk, wheat, peanut, shrimp and crab) and soybeans has not been observed.
(2) False positive results may be obtained due to non-specific reactions in the presence of viscous food or very high of concentrations of protein. In such cases, dilute the sample to an appropriate concentration before conducting the test.
(3) Pressurized and heated food packaged in containers, canned food, and food in retort pouch packaging may exhibit false negative results.
(4) When the sample solution contains a high concentration of buckwheat protein, an obscure line or no line may be observed in the smear test. In such cases, dilute the sample to an appropriate concentration before conducting the test.

Kit component

Components
A: Test strip・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・・ 2-Test × 10 Packs
B: Dilution Buffer・・・・・・・・・・・・・・・・・・・・・・・・・・ 50mL×1 Bottle
C: Extraction buffer (1/10 concentration)・・・・ 100mL×1 Bottle
D: Instruction manual・・・・・・・・・・・・・・・・・・・・・ 1 Sheet
E: Plastic pouched bag・・・・・・・・・・・・・・・・・・・・・ 1 Bag

Illustration of Test plate and the Principle of assay

1) Part names
NPH_NFS001_02.gif
a. Sample solution drop section (Be careful not to touch this section with your finger.)
b. Reagent-containing section
c. Detecting section (Be careful not to scratch this section and touch this section with your finger.)
d. Absorbent pad
e. Measurement items listing position
f.  Test line appearance position (Approx.30mm from the sample solution drop section.)
g. Control line appearance position (Approx.38mm from the sample solution drop section)

2) Detection mechanism
NPH_NFS001_03.gif

NPH_NFS001_04.jpg

Product List

Product Name Cat# Quantity Price

FASTKIT SLIM EGG

NPH-NFS001 20TEST

¥ 32,000
$ 427
€ 320

FASTKIT SLIM MILK

NPH-NFS002 20TEST

¥ 32,000
$ 427
€ 320

FASTKIT SLIM WHEAT

NPH-NFS003 20TEST

¥ 32,000
$ 427
€ 320

FASTKIT SLIM BUCKWHEAT

NPH-NFS004 20TEST

¥ 32,000
$ 427
€ 320

FASTKIT SLIM PEANUT

NPH-NFS005 20TEST

¥ 32,000
$ 427
€ 320

FASTKIT SLIM SOYBEAN

NPH-NFS006 20TEST

¥ 32,000
$ 427
€ 320

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.