PRODUCT CATEGORY

PRODUCT SEARCH

NEWSLETTER

Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

E. coli Ribonuclease H (RNase H), Recombinant

Background

Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.

Application

• Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA (1, 2)
• Removal of poly (A) tails from mRNA after hybridization with oligo (dT) (3)
• Oligodeoxyribonucleotide-directed site-specific cleavage of RNA

Product List

Product Name Cat# Quantity Price

Ribonuclease H

BAM-02-060-EX 1000UNIT

¥ 11,000
$ 147
€ 110

Ribonuclease H

BAM-02-060-5EX 5*1000UNIT

¥ 33,000
$ 440
€ 330

References

1) Gubler U (1987) “Second-strand cDNA synthesis: mRNA fragments as primers.” Method Enzymol 152:
330-335 PMID: 3309563

2) Sambrook J & Russell DW (2001) Molecular Cloning, Chapter 11 “Preparation of cDNA Libraries and Gene
Identification”. CSHL Press

3) Vournakis JN et al (1975) “Electrophoretic patterns of deadenylylated chorion and globin mRNAs.”

Proc.Natl.Acad.Sci.USA 72: 2959-2963 PMID: 1059086

4) Donis-Keller H (1979) “Site specific enzymatic cleavage of RNA.” Nucleic Acids Res. 7: 179-192 PMID:
386279

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.