There are hematopoietic stem cells and bone marrow stromal cells in bone marrow. Bone marrow stromal cells contain undifferentiated mesenchymal stem cells that can differentiate into a variety of cell types such as osteoblasts chondrocytes adipocytes and so on. Osteogenesis Culture Kit (Mouse) (PMC-OGC11-COS) contains cryopreserved cells isolated from mouse bone marrow and two types of culture medium.
The cells in this product can be grown using Growth Medium (Code No. PMC- OGCMG-COS), and then can be differentiated into mature osteoblasts, which form calcified nodules, using Culture Medium (Mouse) (Code No. PMC- OGCMO-COS).
Fig. 1 Morphology of cultured cells
A: Day 0
C: 3 weeks after medium is changed into “Osteogenesis Culture Medium for Osteogenesis Culture kit” (35 mm dish)
|Product Name||Size||Quantity||Storage Conditions||Stability|
|Bone marrow stromal cell, ICR Mouse||1x 106 cells||1||Liquid Nitrogen||1 years|
|125 mL||1||-20°C Freezer||6 months|
|250 mL||1||-20°C Freezer||6 months|
Components of Media Components of Media:
* Growth medium for Osteogenesis Culture kit is sterile, liquid basal medium (α-MEM) which contain contains 10 % FBS, 10 units/ml penicillin and 10 μg/ml streptomycin.
** Culture medium for Osteogenesis Culture kit (Mouse) (Code No. PMC- OGCMO-COS) is sterile, liquid basal medium (α-MEM) which contain contains 10 % FBS, 10 units/ml penicillin, 10 μg/ml streptomycin, dexamethasone, ascorbic acid and β-Glycerophosphate.
Cells are cultured according to protocol and subjected to activity staining of alkaline phosphatase or calcified nodules. The activity of alkaline phosphatase is detected by Alkaline Phosphatase Staining kit (Code: PMC-AK20-COS) and the calcium deposit is detected by Calcified nodule Staining kit (Code: PMC-AK21-COS).
Osteogenesis Culture kit
Osteogenesis Growth Medium
Osteogenesis Culture Medium
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.