Epidimal Adipocyte Culture Kit, H-1 (Mouse) (PMC-EAC11-COS) contains preadipocytes isolated from mouse epididymal adipose tissues and culture medium that induces differentiation of precursor cells into mature adipocytes. The kit provides a convenient system for studying the mechanism of adipogenesis as well as for examining effectiveness of drugs on metabolic syndrome such as obesity, diabetes and hypertension.
|Product Name/Code No.||Size||Quantity||Storage Conditions||Shelf Life|
|Epididymal Preadipocytes, mouse||1.5 x 106 cells||1||Liquid Nitrogen||1 year|
|Epididymal Adipocyte Culture Medium
|125 mL||1||-20°C Freezer||6 months|
One kit contains preadipocytes for half of a 24-well plate.
1. Thaw Epidimal Adipocyte Culture Medium in a 37°C water bath with gentle shaking.
2. Quickly place Common Precursor Cell vial in a 37°C water bath until the contents are thawed.
3. Transfer thawed cells into a 15 mL centrifuge tube containing 10 mL of Epidimal Adipocyte Culture Medium. Mix gently and centrifuge at 4°C at 170 x g for 5 minutes.
4. Remove the supernatant, and re-suspend cells in 10 mL of Epidimal Adipocyte Culture Medium and centrifuge for 5 minutes at 4°C at 170 x g for 5 minutes.
5. Remove the supernatant, and re-suspend the cell pellet in 6.2 mL of Epidimal Adipocyte Culture Medium.
6. Dispense 0.5 mL of cell suspension to each well of 24-well plate and incubate the plate at 37°C in a 5% CO2 humidified incubator.
7. Gently add 0.5 mL of Epidimal Adipocyte Culture Medium to each well of 24-well plate in the following day.
8. Change the medium every other day. Be careful to not disturb the cell layer.
(1) Approximately 3-4 days of culture, preadipocyte culture becomes confluent.
(2) Approximately 5 days of culture, cells turn to mature adipocytes.
(3) Approximately 8 days of culture, cells become hypertrophic and start detaching from the bottom of the well.
Epididymal Adipocyte Culture Medium
> Yoshida, H., Takamura, N., Shuto, T., Ogata, K., Tokunaga, J., Kawai, K., Kai, H. The Citrus Flavonoids Hesperetin and Naringenin Block the Lipolytic Actions of TNF-a in Mouse Adipocytes. Biochem. Biophys. Res. Commun. 394, 728-732 (2010)
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.