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Useful for analyzing effects of drugs on metabolic syndrome such as obesity, diabetes and hypertension

Epididymis Around Adipocyte (Mouse / Rat) and Culture Medium

Background

Epidimal Around Adipocyte (Mouse/Rat) contains preadipocytes isolated from mouse/rat epididymal adipose tissues. And the culture medium induces differentiation of precursor cells into mature adipocytes. These prodcucts are convenient for studying the mechanism of adipogenesis as well as for examining effectiveness of drugs on metabolic syndrome such as obesity, diabetes and hypertension.

Specifications

Product Name/Code No. Size Quantity Storage Conditions Shelf Life
Epididymal Preadipocytes, mouse
(Code: PMC-EAC11C-COS)
1.5 x 106 cells 1 Liquid Nitrogen 1 year
Epididymal Preadipocytes, rat
(Code: PMC-EAC01C-COS)
3 x 106 cells 1 Liquid Nitrogen 1 year
Epididymal Adipocyte Culture Medium
(Code: PMC-EACMR-COS)
250 mL 1 -20°C Freezer 6 months

Protocol: Culture with 24-well plate

One kit contains preadipocytes for half of a 24-well plate.

1. Thaw Epidimal Adipocyte Culture Medium in a 37°C water bath with gentle shaking.
2. Quickly place Common Precursor Cell vial in a 37°C water bath until the contents are thawed.
3. Transfer thawed cells into a 15 mL centrifuge tube containing 10 mL of Epidimal Adipocyte Culture Medium. Mix gently and centrifuge at 4°C at 170 x g for 5 minutes.
4. Remove the supernatant, and re-suspend cells in 10 mL of Epidimal Adipocyte Culture Medium and centrifuge for 5 minutes at 4°C at 170 x g for 5 minutes.
5. Remove the supernatant, and re-suspend the cell pellet in 6.2 mL of Epidimal Adipocyte Culture Medium.
6. Dispense 0.5 mL of cell suspension to each well of 24-well plate and incubate the plate at 37°C in a 5% CO2 humidified incubator.
7. Gently add 0.5 mL of Epidimal Adipocyte Culture Medium to each well of 24-well plate in the following day.
8. Change the medium every other day.  Be careful to not disturb the cell layer.
    (1) Approximately 3-4 days of culture, preadipocyte culture becomes confluent.
    (2) Approximately 5 days of culture, cells turn to mature adipocytes.
    (3) Approximately 8 days of culture, cells become hypertrophic and start detaching from the bottom of the well.

Cellular Morphology

Product List

Product Name Cat# Quantity Price

Epididymis Around Adipocyte

PMC-EAC11C-COS 1VIAL

¥ 115,000
$ 1534
€ 1150

Epididymis Around Adipocyte

PMC-EAC01C-COS 1VIAL

¥ 120,000
$ 1600
€ 1200

Epididymal Adipocyte Culture Medium

PMC-EACMR-COS 250ML

¥ 31,200
$ 416
€ 312

References

> Yoshida, H., Takamura, N., Shuto, T., Ogata, K., Tokunaga, J., Kawai, K., Kai, H. The Citrus Flavonoids Hesperetin and Naringenin Block the Lipolytic Actions of TNF-a in Mouse Adipocytes. Biochem. Biophys. Res. Commun. 394, 728-732 (2010)

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.