Acidic Proteins that regulate bone calcification such as Osteocalcin, Osteopontin and BSP II, are major components of bones and teeth.These acidic proteins are difficult to detect by conventional staining methods of SDS-PAGE gels.
The Stains All Gel Staining Kit is specifically designed to stain strongly acidic proteins.The color of the protein band varies depending on the Protein's isoelectric point and chemical modifications like glycosylation and phosphorylation
|Staining Stock Solution (×10)||40 mL||Room temperature|
|Dilution Buffer||200 mL × 2||Room temperature|
One kit can stain 20 mini slab gels
#Additional Materials Required
Step to stain 1 mini slab gel
1. Wash the SDS-PAGE gel in 20~30 ml of 25% isopropanol. Shake for 20 minutes.
2. Decant the isopropanol and repeat step1 (×3 times or more) to completely remove SDS. Overnight washing is recommended for final step, because any remaining SDS in the gel will react with the stain solution and interfere with protein staining.
3. Decant 25% isopropanopl and wash gel in deionized water for 10 minutes with shaking.
4. Replace the water and repeat step 3 for 3 times.
5. Decant water and add 20 ml of prepared Stain Solution.
6. Cover dish with aluminum foil to protect from light. Shake for 3 hours.
7. Decant Stain Solution and wash the gel in deionized water at least 2 times. The red color in the background will fade if the gel is left under natural light for approximately 10 minutes. The protein bands of various staining intensities and colors will become more visible
|M: Molecular-weight marker Co I : Type I Collagen Co II : Type II Collagen BSA : Bovine serum albmin||HM: Human milk total protein OPN: Osteopontin from human milk BSP II : Bovine bone sialoprotein MGP: Bovine matrix glaprotein|
Stains All Gel Staining Kit
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.