PRODUCT CATEGORY

PRODUCT SEARCH

NEWSLETTER

Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

Useful for the staining of Tartrate-Resistant Acid Phosphatase in osteoclasts.

TRAP Staining Kit

Background

The TRAP Staining Kit is used for the staining of Tartrate-Resistant  Acid Phosphatase in osteoclasts. Bone mass is controlled by the balance between the activity of osteoblasts bone formation and the activity of osteoclasts.
Alkaline phosphatase (ALP staining kit, Cat.No.PMC-AK20-COS) and tartrate-resistant acid phosphate are used as markers for osteoblasts and osteoclasts, respectively


Figure 1: TRAP staining of Osteoclast

Kit component

Component Quantity Storage
Tartrate-containing Buffer 50 mL 4C (Do not freeze)
Chromogenic Substrate 10 vials 4C

One kit contains regents for 10 × 96-well plates

#Additional Materials Required
 ・10% Formalin Neutral Buffer Solution
 ・Phosphate Buffered Saline (PBS)
 ・Distilled or deionized water

 

  Frequently Asked Questions

Protocol (96-well plate format)

1. Remove culture medium. Wash each well once with 100uL of PBS.
2. Add 50uL of the 10% Formalin Neutral Buffer Solution to each well and fix for 5 minutes at room temperature.
3. Wash each well with 250uL of deionized water. (× 3 times)
4. Dissolve 1 vial of Chromogenic Substrate with 5mL of Tartrate-containing Buffer.
5. Add 50uL of Chromogenic Substrates to each well.
6. Incubate at 37℃ for 20-60 minutes. Adjust incubation time until stained TRAP is clearly showing the result in figure 1.
7. Wash with deionized water to stop the reaction.

Note: Excess incubation will be cause of over stained.

 

  Frequently Asked Questions

Product List

Product Name Cat# Quantity Price

TRAP Staining Kit

PMC-AK04F-COS 10PLATE

¥ 33,600
$ 448
€ 336

References
  • Sunao Takesita, Keisuke Kaji, Akira Kudo. Identification and Characterization of the New Osteoclast Progenitor with Macrophage Phenotypes Being Able to Differentiate into Mature Osteoclasts. JOURNAL OF BONE AND MINERAL RESEARCH Volume 15, Number 8 (2000). 1477-1488.
  • BEN A. A. SCHEVEN, JONE S. MILNE, SIMON P. ROBINS. A SEQUENTIAL CULTURE APPROACH TO STUDY OSTEOCLAST DIFFERENTIATION FROM NONADHERENT PORCINE BONE MARROW CELLS. In Vitro Cell. Dev. Biol. July-August (1998). Animal 34: 568-577.
  • Martha J. Somerman, Janice E. Berry, Zhila Khalkhali-Ellis, Philip Osdoby, Robert U. Simpson. Enhanced Expression of α v Integrin Subunit and Osteopontin during Differentiation of HL-60 Cells along Monocytic Pathway. EXPERIMENTAL CELL RESEARCH 216, 335-341(1995).
  • Ichiro Itonaga, Afsie Sabokbar, Susan D. Neale, Nicholas A. Athanasou. 1,25-Dihydroxyvitamin D3 and Prostaglandin E2 Act Directly on Circulating Human Osteoclast Precursors. Biochemical and Biophysical Research Communications 264, 590-595(1999).
  • Hiroshi Takayanagi, Kouetsu Ogasawara, Shigeaki Hida, Tomoki Chiba, Shigeo Murata, Kojiro Sato, Akinori Takaoka, Taeko Yokochi, Hiromi Oda, Keiji Tanaka, Kozo Nakamura and Tadatsugu Taniguchi. T-cell-mediated regulation of osteoclastogenesis by signaling cross-talk between RANKL and IFN- α Nature, Vol. 408, No.6812, 600-605, 30 November 2000.
  • Morinobu, A., Biao, W., Tanaka, S., Horiuchi, M., Jun, L., Tsuji,G., Sakai, Y., Kurosaka, M., Kumagai, S. (-)-Epigallocatechin-3-Gallate Suppresses Osteoclast Differentiation and Ameliorates Experimental Arthritisv in Mice. Arthritis Rheum. 58, 2012-2018 (2008)
  • Hase, H., Kanno, Y., Kojima, H., Sakurai, D., Kobata, T. Coculture of Osteoclast Precursors With Rheumatoid Synovial Fibroblasts Induces Osteoclastogenesis via Transforming Growth Factor beta-Mediated Down-Regulation of Osteoprotegerin. Arthritis Rheum. 58, 3356-3365 (2008)
  • Cao, H., Kuboyama, N. A Biodegradable Porous Composite Scaffold of PGA/beta-TCP for Bone Tissue Engineering. Bone. 46, 386-395 (2010)
  • Okada, Y., Hamada, N., Kim, Y., Takahashi, Y., Sasaguri, K., Ozono, S., Sato, S. Blockade of Sympathetic beta-receptors Inhibits Porphyromonas Gingivalis-induced Alveolar Bone Loss in an Experimental Rat Periodontitis Model. Arch. Oral Biol. 55, 502-508 (2010)
  • Okamoto, A., Ohnishi, T., Bandow, K., Kakimoto, K., Chiba, N., Maeda, A., Fukunaga, T., Miyawaki, S., Matsuguchi, T. Reduction of Orthodontic Tooth Movement by Experimentally Induced Periodontal Inflammation in Mice. Eur. J. Oral Sci. 117, 238-247 (2009)

Cell Assay Related Research Kit