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Mouse C-peptide EIA

   Back to "Quantitative determination kits for Biomarkers"

Introduction

This enzyme immunoassay (EIA) kit is a stable and convenient assay system for mouse C-peptide (mouse C-peptide I+II) in its serum and plasma. The processing of proinsulin, which occurs within the pancreatic B cell, yields insulin and C-peptide. The insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function. This EIA kit includes synthetic mouse C-peptide II as standard antigen and biotinylated mouse C-peptide II as labeled antigen. The kit also contains specific polyclonal antibody recognizing mouse C-peptide I and mouse C-peptide II equivalently. We have already developed EIA kits which can measure specifically mouse C-peptide I (YII-YK011-EX) and C-peptide II (YII-YK012-EX), respectively and now mouse C-peptide (I+II) kit (YII-YK013-EX) is developed for measuring C-peptide I and C-peptide II together. Namely this kit is useful for measuring total C-peptide level in mouse blood.

Characteristics

This EIA kit is used for quantitative determination of total mouse C-peptide, i.e. C-peptide I+II, in its serum and plasma samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it is not influenced by other constituents in samples. Standard antigen, mouse C-peptide II of this kit is a highly purified synthetic product (purity: higher than 98%).

<Specificity>
This EIA kit shows 93.9% crossreactivity to mouse C-peptide I, 100% to mouse C-peptide II, 22.9% to rat C-peptide and 48.2% to rat C-peptide II. It shows below 2% crossreactivity to mouse insulin and no crossreactivity to human, dog and pig C-peptide.

<Test Principle>
This EIA kit for determination of mouse C-peptide in serum and plasma is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse C-peptide (I+II) and biotin-avidin affinity system. To the wells of the plate coated with goat anti rabbit IgG, labeled antigen, standard antigen or samples and rabbit anti mouse C-peptide antibody are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptavidin (SA) is added to form HRP labeled SA-biotinylated antigen-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3’5,5’-tetramethyl benzidine (TMB) and the concentration of mouse C-peptide is calculated.

Product List

Product Name Cat# Quantity Price

C-peptide1 EIA

YII-YK011-EX 1KIT

¥ 76,000
$ 1014
€ 760

References
  • Wahren, J et al: C-peptide makes a comeback. Diabetes Metab Res Rev, 19, 345-347, 2003
  • Pierson, CR et al: Proinsulin C-peptide replacement in type1 diabetic BB/Wor-rats prevents deficits in nerve fiber regeneration.
    J Neuropathol Exp Neurol, 62, 765-779, 2003
  • Li, ZG et al: C-peptide enhances insulin-mediated cell growth and protection against high glucose-induced apoptosis in SH-SY5Y cells.
    Diabetes Metab Res Rev, 19, 375-385, 2003
  • Johansson, J et al: Molecular effects of proinsulin C-peptide
    Biochem Biophy Res Comm, 295, 1035-1040, 2002
  • Lindon, H et al: C-peptide exerts cardioprotective effects in myocardical ischemia-reperfusion
    Am J Phsiol Heart Circ Physiol, 279, 1453-1459, 2000
  • Matsuda, K and Oka, Y㸸C-peptide (CPR). Nippon Rinsho Vol 57, 313-316, 1999
  • Wentworth, BM et al: Characterization of the two nonallelic genes encoding mouse preproinsulin
    J Mol Evol, 23, 305-312, 1986