This enzyme immunoassay (EIA) kit is a stable and convenient assay system for mouse C-peptide (mouse C-peptide I+II) in its serum and plasma. The processing of proinsulin, which occurs within the pancreatic B cell, yields insulin and C-peptide. The insulin and C-peptide are secreted in equimolar quantities into blood circulation. Therefore, the measurement of C-peptide in blood reflects the concentration of insulin and also provides valuable information to evaluate the pancreatic B cell function. This EIA kit includes synthetic mouse C-peptide II as standard antigen and biotinylated mouse C-peptide II as labeled antigen. The kit also contains specific polyclonal antibody recognizing mouse C-peptide I and mouse C-peptide II equivalently. We have already developed EIA kits which can measure specifically mouse C-peptide I (YII-YK011-EX) and C-peptide II (YII-YK012-EX), respectively and now mouse C-peptide (I+II) kit (YII-YK013-EX) is developed for measuring C-peptide I and C-peptide II together. Namely this kit is useful for measuring total C-peptide level in mouse blood.
This EIA kit is used for quantitative determination of total mouse C-peptide, i.e. C-peptide I+II, in its serum and plasma samples. The kit is characterized by its sensitive quantification and high specificity. In addition, it is not influenced by other constituents in samples. Standard antigen, mouse C-peptide II of this kit is a highly purified synthetic product (purity: higher than 98%).
This EIA kit shows 93.9% crossreactivity to mouse C-peptide I, 100% to mouse C-peptide II, 22.9% to rat C-peptide and 48.2% to rat C-peptide II. It shows below 2% crossreactivity to mouse insulin and no crossreactivity to human, dog and pig C-peptide.
This EIA kit for determination of mouse C-peptide in serum and plasma is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse C-peptide (I+II) and biotin-avidin affinity system. To the wells of the plate coated with goat anti rabbit IgG, labeled antigen, standard antigen or samples and rabbit anti mouse C-peptide antibody are added for competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP) labeled streptavidin (SA) is added to form HRP labeled SA-biotinylated antigen-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by 3,3’5,5’-tetramethyl benzidine (TMB) and the concentration of mouse C-peptide is calculated.