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The new biomarker in the cranial nerve and food reserch field !

D-Serine Colorimetric Assay Kit

<Advantages>

1. Enzymatic reaction with colorimetric detection for reading on standard UV/VIS absorbance microplate readers (340 nm).
2. Suitable for large numbers of samples.
3. Quantitative for D-serine detection. Detection range : 0.01 mM-1mM

Background

D-Serine Colorimetric Assay Kit D-form amino acids have long been known as components of bacterial cell wall peptidoglycan layers. More recent  developments in analytical technologies have demonstrated that D-amino acids are also present in mammals and  display specific and important physiological activities. Particularly high levels of D-serine levels are present in brain  tissue where D-serine functions as an important co-agonist of N-methyl-D-aspartate (NMDA) receptors (NMDR),  involved in regulating higher brain function such as memory and learning. D-serine is suggested to play a role in  neurodegeneration associated with diseases such as Alzheimer's disease (AD) and amyotrophic lateral sclerosis  (ALS). High levels of free D-serine can also be found in human urine although its significance is unclear.
 D-serine is commonly assayed by HPLC or GC following conversion of D-serine to diastereomer derivatives. Such  methods are time consuming, require expensive instrumentation, and are not suitable for processing large  numbers of samples. The D-Serine Colorimetric Assay Kit employs the D-form specific enzyme D-serine  dehydratase from Saccharomyces cerevisiae ( DsdSC ) enabling the quantitation of D-serine by  spectrophotometric measurement.

Kit component

Components Volume Quantity Storage
Assay Buffer 11 mL 2 vials 4°C
LDH Diluent Buffer 3 mL 2 vials
10mM D-Serine Solution 2 mL 2 vials
NADH Solution (20mM)
*Green Cap Tube
200 μL 2 vials
DsdSC Solution *Red Cap Tube
110 μL 1 vial
LDH Stock Solution *Blue Cap Tube 220 μL 1 vial
96-wellplate 96-well 1 vial

Calibration Curve

Calibration Curve

Product List

Product Name Cat# Quantity Price

D-Serine Colorimetric Assay Kit

CSR-CT-DSC-K01E 1KIT

¥ 60,000
$ 800
€ 600

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.