1. Enzymatic reaction with colorimetric detection for reading on standard UV/VIS absorbance microplate readers (340 nm).
2. Suitable for large numbers of samples.
3. Quantitative for D-serine detection. Detection range : 0.01 mM-1mM
D-form amino acids have long been known as components of bacterial cell wall peptidoglycan layers. More recent developments in analytical technologies have demonstrated that D-amino acids are also present in mammals and display specific and important physiological activities. Particularly high levels of D-serine levels are present in brain tissue where D-serine functions as an important co-agonist of N-methyl-D-aspartate (NMDA) receptors (NMDR), involved in regulating higher brain function such as memory and learning. D-serine is suggested to play a role in neurodegeneration associated with diseases such as Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS). High levels of free D-serine can also be found in human urine although its significance is unclear.
D-serine is commonly assayed by HPLC or GC following conversion of D-serine to diastereomer derivatives. Such methods are time consuming, require expensive instrumentation, and are not suitable for processing large numbers of samples. The D-Serine Colorimetric Assay Kit employs the D-form specific enzyme D-serine dehydratase from Saccharomyces cerevisiae ( DsdSC ) enabling the quantitation of D-serine by spectrophotometric measurement.
|Assay Buffer||11 mL||2 vials||4°C|
|LDH Diluent Buffer||3 mL||2 vials|
|10mM D-Serine Solution||2 mL||2 vials|
|NADH Solution (20mM)
*Green Cap Tube
|200 μL||2 vials|
|DsdSC Solution *Red Cap Tube
||110 μL||1 vial|
|LDH Stock Solution *Blue Cap Tube||220 μL||1 vial|
D-Serine Colorimetric Assay Kit