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For respiratory tract research i.e lung disorders!

Rat/Mouse Surfactant Protein D ELISA Kit


Rat/Mouse Surfactant Protein D ELISA Kit [PDF]

Surfactant protein -D (SP-D) is a glycoprotein belonging to the collectin subgroup of  C type lectin produced mainly with an alveolus type II epithelium cell. It has been reported that SP-D plays an important role in natural immunity of living body defense and lipid metabolism as a physiology.

Moreover, human serum SP-D showed a high value in interstitial pneumonitis patients, and reported that it is useful as a marker for diagnoses of interstitial pneumonitis. Therefore, it is being widely used for serum examination of the respiratory field.

Background

This kit is based on sandwich ELISA which can measure the concentration of rat/mouse surfactant protein D (SP-D) using two monoclonal antibodies.

Application

Measurement of Rat/Mouse SP-D

Feature and Advantages

• High Sensitivity: The measurment range is 0.47 - 30 ng/mL.
• Quick Assay: The reaction time is within 4 hours.
• The concentration of SP-D in serum or bronchoalveolar lavage fluid (BALF) can be measured.

Kit component




1. Antibody Coated Plate (96 microwells plate, 8wells x 12 strips)...1 set
2. Rat SP-D Standard 1 (0.47 ng/mL, 0.5mL)...1 vial
3. Rat SP-D Standard 2 (1.88 ng/mL, 0.5mL)...1 vial
4. Rat SP-D Standard 3 (7.5 ng/mL, 0.5mL)...1 vial
5. Rat SP-D Standard 4 (30 ng/mL, 0.5mL)...1 vial
6. Sample Diluent (50mL)...1 vial
7. Concentrated Washing Solution (50mL)...1 vial
8. Enzyme Conjugate (0.15mL)...1 vial
9. Enzyme Conjugate Diluent (15mL)...1 vial
10. Color Developing Reagent A (11mL)...1 vial
11. Color Developing Reagent B (0.5mL)...1 vial
12. Stop Solution (11mL)...1 vial

Assay Procedure

1. Add 100 uL of standard and samples
2. Incubate at 20-30C for 2 hours
3. Wash plate 3 times
4. Add 100 uL of HRP cinj. antibody solution
5. Incubate at 20-30C for 1 hour
6. Wash plate 3 times
7. Add 100 uL of color reagent
8. Incubate at 20-30C for 15 minutes
9. Add 100 uL of stop solution

Serum SP-D and BALF Concentrations of Bleomycin-treated Wistar Rats

Serum SP-D and BALF Concentrations of Bleomycin-treated Wistar Rats

Bleomycin (1 mg/kg) was administered intratracheally to male Wistar Rats (8 weeks-old). Serum and bronchoalveolar lavage fluid (BALF) samples were collected from day 0 to 28 after bleomycin treatment. Serum SP-D concentrations of bleomycin-treated rats were elevated from day 5 and peaked on day 10 , then gradually decreased until day 28. Serum samples and BALF samples in control rats were not elevated. In the case of serum aspartate aminotransferase and C-reactive protein, there was no significant difference between bleomycin-treated rats and control rats.

Histological Evaluation

Histological Evaluation

Lungs prepared from normal rats and bleomycin-treated rats were fixed in 20% formalin solution and embedded in paraffin. These samples were stained with hematoxylin and eosin for histological evaluation. Inflammatory cell infiltration and pulmonary edema were observed in cell wall after day 3. On day 10 when serum SP-D concentration of bleomycin-treated rats were peaked, the density of inflammatory cell infiltration was the highest of other points and alveolar collapse was recognized. After day 28, SP-D concentration of serum and BALF were decreased and inflammatory cell infiltration and alveolar collapse were improved. These results showed that concentration of serum SP-D in bleomycin-treated rats corresponds with inflammation in lung.

Product List

Product Name Cat# Quantity Price

Rat/Mouse Surfactant Protein D ELISA Kit

YMS-80072-EX 96TEST

¥ 192,000
$ 2560
€ 1920

Related Products;
Anti Surfactant Protein D (12A2) [Cat. No. YMS-80073]
Anti Surfactant Protein D (5F4) [Cat. No. YMS-80075] 

References

• Murata M. et al., Exp Lung Res. 36 463-468 (2010)
• Murata M. et al., J Toxical Sci. 35 Supplement P.S208

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.