PRODUCT CATEGORY

PRODUCT SEARCH

NEWSLETTER

Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

For research of skeletal metabolism

Bone Resorption Assay Kit

Background

This product is an assay kit to measure bone resorption activity with a fluoresceinated calcium phosphate-coated plate (patent pending).  The coated calcium phosphate is first bound to fluoresceinamine-labeled chondroitin sulfate (FACS), which is released from the calcium phosphate layer into conditioned medium by osteoclastic resorption activity.  Bone resorption activity is evaluated by measuring the fluorescence intensity of the conditioned medium.  Unlike traditional pit assays, rapid evaluation is possible with this system .


BONE RESORPTION ASSAY KIT 24

BONE RESORPTION ASSAY KIT 48

 


BONE RESORPTION ASSAY KIT Flyer Download [PDF]

Principle

Features

1) Bone resorption activity is evaluated simply by measuring the fluorescence intensity of the medium.
2) The fluorescence excitation and emission wavelengths are identical to those for the commonly-used fluorescent dye, FITC.
3) Microscopic observation of cell morphology is possible.
4) Pit area analysis is also possible after removing the cells.

Ready to use, sterile components.

Application

[Differentiation into osteoclast by RANKL]


Osteoclastic differentiation of RAW264 cells induced by RANKL (100 ng/mL) was evaluated by measuring the fluorescence intensity of the conditioned medium.  Photographs show pit formation on the calcium phosphate (CaP) coating with or without RANKL stimulation.(●: with RANKL, ○: without RANKL, *: p<0.05, **: p<0.001)

 

[Comparison between fluorescence intensity and pit area]


RANKL-dependent increase of fluorescence intensity of the conditioned medium (left) and pit area (right) from RAW264 cells in fluoresceinamine-labeled chondroitin sulfate (FACS)/ calcium phosphate (CaP)-coated plates (mean ± SD, n = 3, **: p<0.001).

 

[Drug evaluation]


The inhibitory effects of Pamidronate and β-Estradiol on calcium phosphate resorption induced by RANKL (100 ng/mL) were measured by fluorescence intensity (left) and pit area (right) (mean ± SD, n = 3, *:p<0.05, **: p<0.001).

 

 Method of Measuring Pit Area 

 

Product List

Product Name Cat# Quantity Price

BONE RESORPTION ASSAY KIT 24

CSR-BRA-24KIT 1KIT

¥ 66,000
$ 880
€ 660

BONE RESORPTION ASSAY KIT 48

CSR-BRA-48KIT 1KIT

¥ 73,000
$ 974
€ 730

BONE RESORPTION ASSAY KIT 48x2

CSR-BRA-48X2KIT 2*1KIT

¥ 121,000
$ 1614
€ 1210

BONE RESORPTION ASSAY PLATE 24

CSR-BRA-24P 1PLATE

¥ 30,000
$ 400
€ 300

BONE RESORPTION ASSAY PLATE 48

CSR-BRA-48P 1PLATE

¥ 37,000
$ 494
€ 370

BONE RESORPTION ASSAY PLATE 48x2

CSR-BRA-48X2P 2*1PLATE

¥ 61,000
$ 814
€ 610

BONE RESORPTION ASSAY FACS

CSR-BRA-FACS1 13ML

¥ 37,000
$ 494
€ 370

BONE RESORPTION ASSAY BUFFER

CSR-BRA-B1 10ML

¥ 4,000
$ 54
€ 40

Citation
  • Anti-Osteoclastic Activity of Artemisia capillaris Thunb. Extract Depends upon Attenuation of Osteoclast Differentiation and Bone Resorption-Associated Acidification Due to Chlorogenic Acid, Hyperoside, and Scoparone.
    Lee SH, Lee JY, Kwon YI, Jang HD.
    Int J Mol Sci. 2017 Feb 4;18(2). PMID: 28165389
  • The inhibitory effect of beta-lapachone on RANKL-induced osteoclastogenesis.
    Gu DR, Lee JN, Oh GS, Kim HJ, Kim MS, Lee SH.
    Biochem Biophys Res Commun. 2017 Jan 22;482(4):1073-1079. PMID: 27913299
  • PKCβII-mediated cross-talk of TRPV1/CB2 modulates the glucocorticoid-induced osteoclast overactivity.
    Bellini G, Torella M, Manzo I, Tortora C, Luongo L, Punzo F, Colacurci N, Nobili B, Maione S, Rossi F.
    Pharmacol Res. 2017 Jan;115:267-274. PMID: 27919827
  • Regulation of RANKL-induced Osteoclastogenesis by RING finger protein RNF114.
    Lin B, Ke Q, Leaman DW, Goel V, Agarwal A.
    J Orthop Res. 2017 Jul 14. PMID: 28708287
  • Effect of low-intensity pulsed ultrasound on the activity of osteoclasts: An in vitro study.
    Feres MF, Kucharski C, Diar-Bakirly S, El-Bialy T.
    Arch Oral Biol. 2016 Oct;70:73-78. PMID: 27341458
  • Myeloid thrombomodulin lectin-like domain inhibits osteoclastogenesis and inflammatory bone loss.
    Cheng TL, Lai CH, Shieh SJ, Jou YB, Yeh JL, Yang AL, Wang YH, Wang CZ, Chen CH, Shi GY, Ho ML, Wu HL.
    Sci Rep. 2016 Jun 17;6:28340. PMID: 27311356
  • Lung tumor-associated dendritic cell-derived resistin promoted cancer progression by increasing Wolf-Hirschhorn syndrome candidate 1/Twist pathway.
    Kuo CH, Chen KF, Chou SH, Huang YF, Wu CY, Cheng DE, Chen YW, Yang CJ, Hung JY, Huang MS.
    Carcinogenesis. 2013 Nov;34(11):2600-9. PMID: 23955539
  • Dual inhibition of canonical and noncanonical NF-κB pathways demonstrates significant antitumor activities in multiple myeloma.
    Fabre C, Mimura N, Bobb K, Kong SY, Gorgun G, Cirstea D, Hu Y, Minami J, Ohguchi H, Zhang J, Meshulam J, Carrasco RD, Tai YT, Richardson PG, Hideshima T, Anderson KC.
    Clin Cancer Res. 2012 Sep 1;18(17):4669-81. PMID: 22806876
  • Osteoblastogenesis and osteoprotection enhanced by flavonolignan silibinin in osteoblasts and osteoclasts.
    Kim JL, Kang SW, Kang MK, Gong JH, Lee ES, Han SJ, Kang YH.
    J Cell Biochem. 2012 Jan;113(1):247-59. PMID: 21898547

References
  • Evaluation of osteoclastic resorption activity using calcium phosphate coating combined with labeled polyanion.
    Miyazaki T, Miyauchi S, Anada T, Imaizumi H, Suzuki O.
    Anal Biochem. 2011 Mar 1;410(1):7-12. PMID: 21078287
  • Effect of chondroitin sulfate-E on the osteoclastic differentiation of RAW264 cells.
    Miyazaki T, Miyauchi S, Tawada A, Anada T, Suzuki O.
    Dent Mater J. 2010 Aug;29(4):403-10. PMID: 20610874
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.
  • BONE RESORPTION ASSAY KIT [CSR-BRA-24KIT, CSR-BRA-48KIT]
    This product is an assay kit for the measurement of bone resorption activity using a fluoresceinated calcium phosphate-coated plate.
  • BONE RESORPTION ASSAY PLATE [CSR-BRA-24P, CSR-BRA-48P]
    This product is a calcium phosphate (CaP)-coated 24-well plate for measuring bone resorption activity by osteoclasts.
  • BONE RESORPTION ASSAY FACS [CSR-BRA-FACS1]
  • BONE RESORPTION ASSAY BUFFER [CSR-BRA-B1]
  • Osteoblast
  • Osteoblast from cranial bone culture kit V-1(Rat) [PMC-OBC02-COS]
  • Osteogenesis Culture kit (Mouse) [PMC-OGC11-COS]
  • Osteoclast Culture kit V-1 (mouse) [PMC-OSC14-COS]
  • Osteoclast Culture kit V-2 (mouse) [PMC-OSC13-COS]
  • Osteoclast Culture kit V-2 (rat) [PMC-OSC12-COS]
  • Osteoclast Culture kit V-4 (rat) [PMC-OSC11-COS]