Western Blot: ~1/2,000
Optimal dilutions/concentrations should be determined by the end user.
Crude membrane fraction (30 ug) prepared from Arabidopsis shoots was subjected
to immunoblotting with the antibody in the absence (lane 1) or presence (lane 2) of
the corresponding antigen peptide at a concentration of 2 ug/ml.
1) Ide, Y., Nagasaki, N., Tomioka, R., Suito, M., Kamiya, T. and Maeshima, M. (2007) Molecular properties of a novel, hydrophilic cation-binding protein associated with the plasma membrane. J. Exp. Botany, 58: 1173-1183.
2) Nagasaki, N., Tomioka, R. and Maeshima, M. (2008) A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the posphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2. FEBS J. 275: 2267-2282.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.