Western Blot: ~1/2,000
Optimal dilutions/concentrations should be determined by the end user.
Lanes 1 and 2: cell extract of E. coli expressing GST-N(MARBP)-His protein (74 kDa).
Lanes 3 and 4: cell extract of E. coli expressing GST-C(MARBP)-His protein (50 kDa).
E. coli was cultured in the presence (lanes 2 and 4) or absence (lanes 1 and 3) of IPTG. These
recombinant MARBP was clearly detected with anti-MARBP.
Immunostaining of nucleoli
Immunolocalization of MARBP in tobacco cells. Tobacco BY-2 cells subcultured for 4 d were
harvested, fixed with formamide, and then treated with anti-NtMARBP (B, D), and control
serum (F) antibodies. The antigen was visualized with Cy3-coupled goat anti-(rabbit IgG)
antibody (B, D, F). The same cells were treated with DAPI to visualize nuclei (A, C, E). Images
of Cy3 and DAPI fluorescence were observed under a fluorescent microscope.
Fujiwara, S., Matsuda, N., Sonobe, S., Maeshima, M. (2002) Molecular properties of a matrix attached region-binding protein located in the nucleoli of tobacco cells. Plant Cell Physiol., 43: 1558-1567.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.