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Complete culture kit for porcine embryos in vitro

This is a complete culture kit for porcine embryos in vitro containing porcine oocyte/embryo collection medium (POE-CM), basic medium for porcine oocyte maturation (POM), porcine fertilization medium (PFM), defined medium for porcine embryos (PZM-5), defined medium for late stage porcine embryos (PBM), dbcAMP concentrated solution-100X (dbcAMP-100X) and Reproplate. This kit can efficiently produce transferable embryos (blastocysts) under a low oxygen condition (5%O2/5%CO2/90%N2, 39℃) without the coculture of cumulus/granulosa cells. This kit can support to increase the blastomere numbers of blastocysts and the formation of hatched blastocysts.

 

Cumulus-oocyte complexes
just after the collection
Denuded embryos Blastocysts

Kit component

1) Porcine oocyte/embryo collection medium (POE-CM) Catalog No.: CK020, 100 ml x 3

2) Basic medium for porcine oocyte maturation (POM) Catalog No.: CK021, 25 ml x 2

3) Porcine fertilization medium (PFM) Catalog No.: CK023, 100 ml

4) Defined medium for porcine embryos (PZM-5) Catalog No.: CK024, 25 ml

5) Defined medium for late stage porcine embryos (PBM) Catalog No.: CK025, 25 ml

6) dbcAMP concentrated solution-100X (dbcAMP-100X) Catalog No.: CK027, 0.3 ml

7) Reproplate, Catalog No.: CK028, 10 plates x 3

Necessary Equipments and Reagents

1) Sterile PBS (-) or saline solution (0.9%)

2) Equine chorionic gonadotropin (eCG; 1,000IU): Yell pharmaceutical co., Ltd.

3) Human chorionic gonadotropin (hCG; 5,000IU): Yell pharmaceutical co., Ltd.

4) Paraffin liquid reagent: Merk chemicals (Cat. No.: 107162)

5) Percoll: GE Healthcare UK Ltd. (17-0891-02)

6) Sperm diluent for in vitro fertilization (SEM-5X): COSMO BIO Co., Ltd (Cat. No.: CK026)

7) Culture dish ・35 mm culture dish: Greiner bio-one (627160) ・Petri Dish: FALCON (1112)

8) Centrifuge tube (15 ml, 50 ml)

9) Disposable syringe (10 ml)

10) Syringe needle (18 G)

11) Pipette

12) Glass capillary pipette

13) Aspiration tube

14) Straw cutter

15) Hemacytometers, Thoma

16) Stereomicroscope

17) Multi-gas incubator

18) Clean bench

19) Centrifuge

20) Thermostat bath

21) Micro thermo plate

22) Vortex mixer

23) Others

Product List

Product Name Cat# Quantity Price

Complete culture kit for porcine embryos in vitro

CSR-CK030 1KIT

¥ 48,000
$ 640
€ 480

Porcine oocyte/embryo collection medium

CSR-CK020 5*100ML

¥ 10,000
$ 134
€ 100

Basic medium for porcine oocyte maturation

CSR-CK021 3*25ML

¥ 16,000
$ 214
€ 160

Porcine fertilization medium

CSR-CK023 2*100ML

¥ 20,000
$ 267
€ 200

Defined Medium for Porcine Embryos(PZM-5)

CSR-CK024 3*25ML

¥ 16,000
$ 214
€ 160

dbcAMP concentrated solution-100X

CSR-CK027 0.5ML

¥ 8,000
$ 107
€ 80

Reproplate

CSR-CK028 10*10PLATE

¥ 47,000
$ 627
€ 470

References

> Mito T, Yoshioka K, Nagano M, Suzuki C, Yamashita S, Hoshi H. Transforming growth factor-??in a defined medium during in vitro maturation of porcine oocytes improves their developmental competence and intracellular ultrastructure. Theriogenology 2009; 72: 841-850.
> Suzuki C, Yoshioka K. Effects of amino acid supplements and replacement of polyvinyl alcohol with bovine serum albumin in porcine zygote medium. Reprod Fertil Dev 2006; 18: 789-795.
> Suzuki C, Yoshioka K, Sakatani M, Takahashi M. Glutamine and hypotaurine improves intracellular oxidative status and in vitro development of porcine preimplantation embryos. Zygote 2007; 15: 317-324.
> Yoshioka K, Suzuki C, Tanaka A, Anas IMK, Iwamura S. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol Reprod 2002; 60: 112-119.
> Yoshioka K, Suzuki C, Itoh S, Kikuchi K, Iwamura S, Rodriguez-Martinez H. Production of piglets derived from in vitro-produced blastocysts fertilized and cultured in chemically defined media: effects of theophylline, adenosine, and cysteine during in vitro fertilization. Biol Reprod 2003; 69: 2092-2099.
> Yoshioka K, Suzuki C, Onishi A. Defined system for in vitro production of porcine embryos using asingle basic medium. J Reprod Dev 2008; 54: 208-213.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.