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Collagen solutions for cell and tissue culturing

AteloCell® Atelocollagen, Native collagen Acidic Solutions
Atelocollagen : Pepsin-solubilized type I collagen solution
Native collagen : Acid-solubilized type I collagen solution

Background

Collagen solution for cell and tissue culturing is highly Purified collagen solution manufactured in KOKEN (Tokyo) advanced technology with excellent quality control.

Cell and tissue cultures with Koken collagen solutions can express cellular functions like in vivo cells. Collagen not only plays the role of physical scaffold for the proliferating cells, but also enhances cell adhesion, proliferation and function. Collagen creates a natural extracellular environment that is important for cell communication and layer formation. The synthesizing capability of albumin, a functions shared among hepatocytes, is maintained at a high level when cultured on plates coated with collagen solution. When skin fibroblasts are cultured three-dimensionally on collagen gel , the whole collagen contracts from the contractility of the cells producing a dermis model that closely resembles the structure of live dermis.


AteloCell flyer download [PDF]

Features and Advantages

1. All products are sterilized. The used collagen are extracted from bovine dermis, solubilized and purified by our original methods.
2. Acidic solutions, I-PC and I-AC, form fibrils when being neutralized at 37°C

Application

# Collagen coating of culture plates
# Three dimensional culture in a collagen gel
# Culture on a gel

Instructions for preparing collagen solutionsPDF

References

1) An In vitro Multistep Carcinogenesis Model for Human Cervical Cancer. Narisawa-Saito M, Yoshimatsu Y, Ohno S, et al. Cancer Res. 2008 Jul 15;68(14):5699-705

2) Proteomic analysis of hypoxia-induced tube breakdown of an in vitro capillary model composed of HUVECs : Potential role of p38-regulated reduction of HSP27. Eguchi R, Naitou H, Kunimasa K, et al.Proteomics. 2008 Jul;8(14):2897-906

3) Construction of multifunctional proteins for tissue engineering: Epidermal growth factor with collagen binding and cell adhesive activities. Hannachi Imen E, Nakamura M, Mie M, Kobatake E. J Biotechnol. 2009 Jan 1;139(1) :19-25

4) Possible involvement of caspase-6 and -7 but not caspase-3 in the regulation of hypoxia-induced apoptosis in tube-forming endothelial cells. Eguchi R, Tone S, Suzuki A, et al., Exp Cell Res. 2009 Jan 15;

Product List

Product Name Cat# Quantity Price

Atelocollagen, Bovine dermis, 3mg/mL

KOU-IPC-30 50ML

¥ 9,000
$ 120
€ 90

Atelocollagen, Bovine dermis, 5mg/mL

KOU-IPC-50 50ML

¥ 13,500
$ 180
€ 135

Native collagen, Bovine dermis, 3mg/mL

KOU-IAC-30 50ML

¥ 14,600
$ 195
€ 146

Native collagen, Bovine dermis, 5mg/mL

KOU-IAC-50 50ML

¥ 23,700
$ 316
€ 237

1) An In vitro Multistep Carcinogenesis Model for Human Cervical Cancer. Narisawa-Saito M, Yoshimatsu Y, Ohno S, et al. Cancer Res. 2008 Jul 15;68(14):5699-705
2) Proteomic analysis of hypoxia-induced tube breakdown of an in vitro capillary model

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

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