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Pin-point Protein Labeling An amber stop codon (UAG) is used for pin-point fluorescence or biotin labeling.

CloverDirect™ tRNA Reagents for Site-Directed Protein Functionalization Details

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Background

CloverDirect tRNA Reagents for Site-Directed Protein Functionalization allow the incorporation of unnatural amino acids at defined positions of proteins using in vitro translation. Unnatural amino acids containing fluorescent groups, biotin, PEG, photo-crosslink, etc are available. Proteins with unnatural amino acids will be obtained within a few hours just by adding CloverDirect reagents and DNA template having an amber stop codon (UAG) or a four-base codon (CGGG) to an in vitro translation system. CloverDirect covers the following four applications. In addition, we provide custom services for the expression of proteins with unnatural amino acids (contact us).

Site-Directed Fluorescence Labeling

It is not easy to incorporate fluorescent groups into proteins in a site-direct and quantitative fashion by chemical modification. CloverDirect tRNA Reagents for Site-Directed Fluorescence Labeling allow the incorporation of fluorescent unnatural amino acids into proteins in a site-direct and quantitative fashion. Various fluorescent dyes are available including those for 488 nm, 543 nm and 633 nm excitation.

Site-Directed Fluorescence Labeling

Site-Directed Biotin Labeling

CloverDirect tRNA Reagents for Site-Directed Biotin Labeling allow the incorporation of biotinylated unnatural amino acids into proteins in a site-direct and quantitative fashion. Labeling proteins are available for the oriented immobilization onto avidin-coated plates and beads. The biotinylated amino acids have one or two aminohexyl liners between amino acid and biotin.

Site-Directed Biotin Labeling

Site-Directed Post-Translational Modification

It is not easy to prepare post-translationally modified proteins (phosphorylation, methylation, etc.). CloverDirect tRNA Reagents for Site-Directed Post-Translational Modification allow the incorporation of modified amino acids into proteins to obtain post-translationally modified proteins in a site-direct and quantitative fashion.

Site-Directed Post-Translational Modification

Site-Directed Unnatural Mutagenesis

By incorporation of unnatural amino acids containing functional groups, novel functional proteins can be designed and synthesized. CloverDirect tRNA Reagents for Site-Directed Unnatural Mutagenesis allow the incorporation of unnatural amino acids with PEG, photo-crosslinking, photo-isomerizable groups, etc.

Site-Directed Unnatural Mutagenesis

Product List

Site-Directed Fluorescence Labeling
Site-Directed Biotin Labeling
Site-Directed Post-Translational Modification
Site-Directed Unnatural Mutagenesis
Product List [PDF]

*Please note that the delivery lead time may be 2 - 3 months if the product is out of stock. Please inquire about the stock status.contact us

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.