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Useful for the performance analysis on chromatin-nucleosome.

Analysis on Cellular Dynamics and Chromatin-nucleosome

Histone Variant Monoclonal Antibodies

Nucleosomes are composed of four different histone proteins designated H2A, H2B, H3, and H4. In humans, five variants of histone H3 are reported: H3.1, H3.2, H3.3, H3t, and CENP-A. The two major Histone H3 variants, H3.1 and H3.3, are the main variants displaying distinct genomic localization patterns in eukaryotes. Deposition of Histone H3.1 is associated with DNA synthesis during DNA replication and possibly DNA repair, while Histone H3.3 is incorporated independently of DNA synthesis and is the predominant form of H3 found in non-dividing cells. Hence, these new Histone H3 variant monoclonal antibodies offer great utility for dissecting the functional significance of these H3 variants and the molecular mechanisms associated with their deposition. Recently, it was shown that a genomic gene cluster regulating skeletal myogenesis is marked by H3.3 protein prior to cellular muscle formation and that H3.3 marking of this region enables myogenic gene activation (Ref. 2). These results suggest that monitoring H3.3 marking at specific loci may be useful in the prediction of cell fate. These H3.3 monoclonal antibodies are expected to be useful probes in the field of regenerative medicine



Histone H3.1, H3.2 and H3.3

Anti Histone H3.1/H3.2 Antibody [clone: 6G3C7] Cat.#CAC-CE-039A
Anti Histone H3.1/H3.2 Antibody [clone: 1D4F2] Cat.#CAC-CE-039B
Anti Histone H3.3 Antibody [clone: 6CV4A3] Cat.#CAC-CE-040A
Anti Histone H3.3 Antibody [clone: 4H2D7] Cat.#CAC-CE-040B


Histone H3 and Modified Histone H3 Antibodies

Histones are the main protein components of chromatin. To facilitate nuclear packaging and control of gene expression, DNA in chromatin is wound around nucleosome particles composed primarily of the Histones H2A, H2B, H3, and H4. Histone N-terminal regions (histone tails) protrude from the nucleosome core and are subject to a variety of reversible, regulated modifications (including acetlylation, phosphorylation, and methylation) influencing transcription and chromatin structure. How such modifications are regulated and how these modifications effect gene expression continues to be an area of intense interest and research. In such studies, chromatin immunoprecipitation (ChIP) is perhaps the most widely used experimental procedure. Due to the inherent variability and limited supply of polyclonal antibodies, well characterized monoclonal antibodies are preferred reagents for ChIP.

The versitile set of anti-histone monoclonal antibodies offered here are therefore highly valuable reagents to your lab's epigenetic toolbox.



None modification

Anti Histone H3 Antibody [clone: MABI0301] Cat.#MCA-MABI0001-100-EX


K4(Lysine 4) residue modification

Anti Monomethyl Histone H3(Lys4) Antibody [clone: MABI0302] Cat.#MCA-MABI0002-100-EX
Anti Dimethyl Histone H3(Lys4) Antibody [clone: MABI0302] Cat.#MCA-MABI0003-100-EX
Anti Trimethyl Histone H3(Lys4) Antibody [clone: MABI0302] Cat. #MCA-MABI0004-100-EX


K9(Lysine 9) residue modification

Anti Histone H3 K9Ac Antibody [clone: 2G1F9] Cat.#CAC-CE-037A
Anti Acetyl Histone H3(Lys9) Antibody [clone: MABI0305] Cat.#MCA-MABI0005-100-EX
Anti Monomethyl Histone H3(Lys9) Antibody [clone: MABI0306] Cat.#MCA-MABI0006-100-EX
Anti Dimethyl Histone H3(Lys9) Antibody [clone: MABI0307] Cat.#MCA-MABI0007-100-EX
Anti Trimethyl Histone H3(Lys9) Antibody [clone: MABI0308] Cat.#MCA-MABI0008-100-EX


K9/27(Lysine 9/27) residue modification

Anti Acetyl Histone H3(Lys9/27) Antibody [clone: MABI0310] Cat.#MCA-MABI0010-100-EX


K27(Lysine 27) residue modification

Anti Acetyl Histone H3(Lys27) Antibody [clone: MABI0309] Cat.#MCA-MABI0009-100-EX
Anti Monomethyl Histone H3 (Lys27) Antibody [clone: MABI0321] Cat.#MCA-MABI0321-100-EX
Anti Trimethyl Histone H3 (Lys27) Antibody [clone: MABI0323] Cat.#MCA-MABI0323-100-EX
Anti Dimethyl Histone H3(Lys27) Antibody [clone: MABI0324] Cat.#MCA-MABI0324-100-EX


K36(Lysine 36) residue modification

Anti Monomethyl Histone H3 (Lys36) Antibody [clone: MABI0331] Cat.#MCA-MABI0331-100-EX
Anti Dimethyl Histone H3 (Lys36) Antibody [clone: MABI0332] Cat.#MCA-MABI0332-100-EX
Anti Trimethyl Histone H3 (Lys36) Antibody [clone: MABI0333] Cat.#MCA-MABI0333-100-EX


S10(Serine10) residue modification

Anti Histone H3 S10ph Antibody [clone: 6G8B7] Cat.#CAC-CE-034A
Anti phospho Histone H3, Ser10 Antibody [clone: MABI0312] Cat.#MCA-MABI0012-100-EX


T11(Threonine11) residue modification

Anti Histone H3 T11ph Antibody [clone: 6G12C5] Cat.#CAC-CE-035A


T11(Threonine11) residue modification

Anti Histone H3 T32ph Antibody [clone: 6C7G12] Cat.#CAC-CE-036A


T32(Threonine32) residue modification

Anti phospho Histone H2B (Ser14) Antibody [clone: MABI0251] Cat.#MCA-MABI0251-100-EX


RNA polymerase 2 Monoclonal Antibodies

RNA polymerase II (RNAPII) transcribes all protein-coding genes and many non-coding genes, and the activity of RNAPII correlates with the phosphorylation state of RPB1, the large catalytic subunit of RNAPII.. RPB1 has an unusual C-terminal domain (CTD) that consists of repeats of the heptapeptide consensus sequence N-Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7-C, of which there are 52 copies in mammals. The amino acids in these repeats are potential targets for modification, such as phosphorylation and glycosylation.



Anti RNA polymerase 2, CTD Ser2ph Antibody [clone: 3E7C7] Cat.#CAC-CE-030A
Anti RNA polymerase 2, CTD Ser5ph Antibody [clone: 1H4B6] Cat.#CAC-CE-031A




Histone Variant Monoclonal Antibodies flyer download [PDF]

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.