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Useful for the primary culture, subculture and tissue culture

CHITOSAN NANOFIBER MATRICES Cell & Tissue Culture Substrates

Background

This product is a cell culture ware that coats the Chitosan Nanofiber. Chitosan is a polysaccharide polymer produced by alkali processing (deacetylation) of chitin, which is natural material found in various living things such as shellfish (shrimp, crab), insects and mushrooms. Unique properties of Chitosan, such as biocompatibility and biodegradability, can be designed by controlling the molecular weight of Chitosan and the degree of deacetylation, conversion rate to the Chitosan.

One of distinguishing properties of Chitosan nanofiber is to become scaffolding material in cell culture. With Chitosan nanofiber, adhesiveness of cultured cell can be improved (compared to the culture in polystyrene plate or glass cover slip).

Chitosan nanofiber is hardly perishable and is an easy-to-use material for cell/tissue culture, compared to poly-L-lysine or collagen coated culture dish.

Feature and Advantages

<Chitosan nanofiber Electrospinning method>

Cell Culture Method

Cell Culture method is one of the most popular experimental method in the medical and biological research.ln recent years, animal experiments are being reduced worldwide, and the needs for alternative methods are increasing.
Scientists and researchers in the basic research and the drug development come to seek for good alternatives,such as improved cell culture methods,innovative high throughput cell culture systems utilizing cell chip, etc.

Chitosan

Chitosan is a polysaccharide polymer produced by alkali processing(deacetylation)of chitin,which is naturalmaterial found in various living things such as shellfish(crab,shrimp),insects and mushrooms. Unique properties of chitosan,such as biocompatibility and biodegradability, can be designed by contorlling the molecularweight of chitosan and the degree of deacetylation,conversion rate to chitosan.

Production Process

Hokkaido Soda produces chitosan from fresh crab,Cbionoecetes japonicus(Red Snow Crab)mainly from its leg shell. Extracted chitosan is converted to nanofiber by the electrospinning method and the fiber is fixed on the coverslips,cell culture plates,etc.

Unique Properties

One of distinguishing properties of chitosan nanofiber is to become scaffolding material in cell culture. With chitosan nanofiber,adhesiveness of cultured cell can be improved(compared to the culture in polystyrene plate or glass cover slip).
Chitosan nanofiber is hardly perishable and is an easy-to-use material for cell/tissue culture,compared to poly-L-lysine or collagen coated culture dish.

Case study

1. Adult mouse DRG culture on Chitosan coated coveslip

Decapitate an adult mouse and cut the skin of the backsection in the middle (a). Separate the dorsal tissue together with dorsal column asa whole(b). Cut the ventral side of the separated tissue,remove the dorsal column and expose the spinal cord under stereomicroscope(c). By removing the spinal cord(d), DRG(dorsal root ganglion)will appear.Retrieve each DRG one by one using tweezers.

Adult mouse dorsal root ganglia(DRG) explants.

DRG did not adhere to the cover slip, on which either collagen type l or poly-L-lysine was applied.  Even it did, the cell adhesivity was very weak, and only slight extension of neurite from the ganglion was observed.
ln the case of liquid chitosan or chitosan nanofiber coated cover slips,cultured tissue adhered firmly to either cover slip.  Both the migration of schwann cells originated from the tissue,and the neurite extension from the ganglion, were very good.
The neurite extension on the coverslip fixed with chitosan nanofiber was prominent; more than 10 times of that of CoIlagen type l or poly-L-lysine coated coversIip, and approximately 6 times of that of liquid chitosan coat coverslip

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2. Cell culture on Chitosan Nanofiber coveslip

Schwann cell IM32 and primary mouse DRG (dispersed) were cultured on chitosan nanofiber.  The affinity of cells to the aligned chitosan nanofiber was very good.  Based on the evaluation of immunofluorescence assay, both schwann cells and neurite of DRG neuron almost grew in line with the axial direction of aligned nanofiber

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3. Rat visceral fat cell culture

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Summaries of product characteristics

<Product information>

WARE KIND QUANTITY PACKAGING STORED EXPIRATION DATE
cover slip 13mmφ 5 individual room temperature 1year
cover slip 15mmφ 5 individual room temperature 1year
plate 12 well plate 1 individual room temperature 1year
plate 24 well plate 1 individual room temperature 1year
plate 96 well plate 1 individual room temperature 1year

<Product specification>

ITEM SPECIFICATION REMARKS
deacetylation of Chitosan >90%
Amount of fixed Chitosan 0.05 - 0.1mg/cm2 For cover slip, Chitosan nanofiber is fixed on one
Sterlization method Ethanol Ware is wet by ethanol.

Product List

Product Name Cat# Quantity Price

Chitosan Nanofiber Coated Culture ware : cover slip,13 mm(PHI)

HKS-HSC13 5SHEET

¥ 2,000
$ 27
€ 20

Chitosan Nanofiber Coated Culture ware : cover slip,15 mm(PHI)

HKS-HSC15 5SHEET

¥ 2,000
$ 27
€ 20

Chitosan Nanofiber Coated Culture ware : plate, 12 well plate

HKS-HSP12 1PLATE

¥ 3,800
$ 51
€ 38

Chitosan Nanofiber Coated Culture ware : plate, 24 well plate

HKS-HSP24 1PLATE

¥ 3,800
$ 51
€ 38

Chitosan Nanofiber Coated Culture ware : plate, 96 well plate

HKS-HSP96 1PLATE

¥ 3,800
$ 51
€ 38

References

Tsuneo Ohkuma ,et al.,Chitin and Chitosan Research12(2) 190-191(2006) 「Preparation of Chitosan Nanofiber Mesh as a Scaffold for Regenerating Nerve Tissue」

Katsumi Konno ,et al.,Chitin and Chitosan Research13(2) 124-125(2007) 「Manufacturing chitosan nanofiber by electrospinning process」

Katsuyoshi Sakai ,et al.,Chitin and Chitosan Research13(2) 126-127(2007) 「Manufacturing cell culture substrates on chitosan nanofiber and manufacturing chitosan gel,chitosan sponge」

Kazuhiko Watabe ,et al.,Chitin and Chitosan Research13(2) 128-129(2007)「Neural tissue culture on chitosan nanofiber matrices」

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

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