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A Multiplex Real-Time PCR (qPCR) Assay for Detection of Chikungunya virus (CHIKV) and Four Dengue virus (DENV) Types

CHIK-DEN 5 - plex qRT - PCR Kit

Background

Both Chikungunya virus (CHIKV) and Dengue virus (DENV) cause acute febrile disease. DENV consists of four serotypes. Different DENV serotypes can lead to varying clinical and epidemiologic profiles. Antibodies developed during primary infection protect against subsequent infection with the same serotype, but can enhance disease following secondary infection with a heterologous DENV serotype. As clinical symptoms of CHIKV infections are similar to DENV infections, good detection methods to identify CHIKV infection are important to improved treatment and clinical management.

Features

  • Possible to detect and discriminate DENV 1-4 and CHIKV at the same time using single tube reaction.
  • Possible to diagnosis quanttatively for DENV 1-4 and CHIKV.

Kit Components

Reagent 25rxns (µL)
2x Reaction mix 375
RT-PCR Enzyme mix *1 15
5plex CDV primer-probe mix *2 30
Internal Control template 5
Water (PCR Grade) 200
    *1 Do not vortex
    *2 Store in the dark at -20 °C

Specificity

Assay was tested negative with the following pathogens (5000cp/reaction): No cross-reaction observed.
  • Chlamydophila Pneumoniae
  • Cytomegalovirus
  • Enterovirus 71
  • Epstein-Barr Virus
  • Hepatitis B Virus
  • Human Immunodeficiency Virus-1
  • Human Herpesvirus 6
  • Influenza swH1N1
  • Influenza H3N2 virus
  • Influenza B virus
  • Mycobacterium Tuberculosis
  • Parvovirus B19
  • Staphylococcus pneumoniae
  • Varicella-zoster virus
  • West Nile virus

Product List

Product Name Cat# Quantity Price

MEASURE CHIK-DEN 5-plex qRT-PCR Kit

UMA-P1D0 1KIT Contact Us

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.