Series of devices are designed for obtaining protein crystals with Counter diffusion method. The series features starter kit and helping assembly effective for protein crystallization.
*What is Counter-diffusion method?
Protein and reservoir solutions diffuse each other from opposite direction through the gel-tube and form concentration gradient along the capillary. It is called “counter-diffusion” method (1)(2). If the precipitant concentration is set higher, wider range of crystallization condition can be scanned. A crystal starts to grow where the concentration of protein and the precipitant is optimized for crystallization. This type of experiment can explore a wide range of crystallization conditions in one single experiment, so that there is high possibility and reproducibility of obtaining high-quality crystals in a single experiment (3)(Fig1).
Kit ComponentCrystal Tube SGT kit
- Sealing Gel Tube (JCB-SGT)
- Gel Tube (10cm)
- Capillary (Φ0.5mm×47mm)
- Down Controller (with or without)
- Heat Controller
- Point Sealer
- SGT Holder
- Silicon Tubing (50cm)
- Sealing Compound
*Low price and high density
JCB-SGT is a high-density crystallization cell used for Japan Aerospace Exploration Agency’ s Protein Crystal Growth Experiment (JAXA PCG).Neither robot nor special device is required for set-up.
Gel tubing (gel in silicone tubing) can make the experimental set-up easy.
*Less volume of protein
2µl / capillary for optimization of crystallization condition and 8µl / capillary for growing crystals for X-ray diffraction experiment.
Crystallization condition can be controlled by the length of gel-tube and concentration of protein and precipitant solutions. The condition is often milder than the one in vapor-diffusion, so that better-quality crystals can be expected.
*High reproducibility / reliability
This device is a spin-off of JAXA-GCF space experiment. High reproducibility and reliability is proven after crystallization over 400 different kinds of proteins.
*Long term stability
Crystals in the capillary are stable for long term. Post-soaking is easy by changing precipitant solution in the cell.
*Acceptable to Membrane proteins
Phase separation due to concentrated detergent in the solution does not occur.Possible to apply to crystalization of membrane proteins.
How to assemble SGT