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Can Get Signal® immunostain Immunoreaction Enhancer Solution

Background

Can Get Signal immunostain has been developed as a reaction-enhancing kit to address the problems often encountered during immunohistological staining and immunostaining of cells. These include inadequate sensitivity, low S/N ratio, and the appearance of nonspecific signals from secondary antibodies. In the past, inadequate detection sensitivity due to low antibody titers was dealt with by increasing the concentration of the added antibody. However, increasing the antibody concentration often caused background and nonspecific responses. By enhancing antigen-antibody reactions using this kit, it will improve the ability to optimize the antibody concentration, lead to clearer staining responses while suppressing nonspecific reactions caused by excessive addition of antibodies.

Feature and Advantages

1: Enhancing signals in immunostaining

  • This solution has effect on enhancing an antigen-antibody reaction, and allows to get clearer signal than the one obtained by conventional methods.
  • It can reduce the amount of secondary antibody used and exposure time in fluorescence/luminescence detection system, accordingly you can have images with high S/N ratio and low background.

2: Compatible with a wide range of antigens and antibodies

  • Two different solutions with different reaction-enhancing effects (Solution A and Solution B) are prepared. Users can select the more suitable one for their target according to the experimental system.

3: Wide versatility

  • It is applicable to all detection systems involving chemical coloration, chemiluminescence and fluorescence because of its composition having no effect on labeling enzymes and fluorescent dyes.
  • It can be used in combination with sensitizing systems such as ABC or polymer-complex method.

Example

Detection of Paxillin by fluorescent antibody immunostaining
(* This data was provided by Dr. Ichiro Harada of Tokyo Institute of Technology)

Fig1 Fig2
Fig.1. Can Get Signal immunostain (Exposure 1 sec, without controlling contrast) Fig.2. TBS (Exposure 3 sec, without controlling contrast)
Fig3  
Fig.3. Merged image of trichrome stain  

Product List

Product Name Cat# Quantity Price

Can Get Signal immunostain Starter Set

TYB-NKB-401 2*5ML

¥ 16,100
$ 215
€ 161

Can Get Signal immunostain Solution A

TYB-NKB-501 1*20ML

¥ 40,100
$ 535
€ 401

Can Get Signal immunostain Solution A

TYB-NKB-501X4 1SET

¥ 93,400
$ 1246
€ 934

Can Get Signal immunostain Solution B

TYB-NKB-601 1*20ML

¥ 40,100
$ 535
€ 401

Can Get Signal immunostain Solution B

TYB-NKB-601X4 1SET

¥ 93,400
$ 1246
€ 934

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.