Syndecan is the family name of transmembrane proteoglycans that carry predominantly heparan sulfate side chains. This proteoglycan family consists of four members. N-syndecan (syndecan-3) is the principal member expressed during early postnatal development in both central and peripheral nervous systems. N-syndecan binds various heparin-binding growth factors such as FGFs via the heparan sulfate moiety, and communicates with the cytoskeleton via the cytoplasmic domain of the core protein. N-syndecan plays a pivotal role in formation of the neural network through these molecular interactions. This antibody recognizes effectively the core protein of N-syndecan.
Characterization of the polyclonal anti-N-syndecan antiserum
Immunoblotting using anti-Nsyndecan of a partially purified preparation of membrane-bound proteoglycan (lane 1), and that digested with chondroitinase ABC (lane 2), and heparitinase I (lane 3). After the heparitinase I treatment , the immunoreactive band is sifted to an apparent molecular weight of approximately 140 kDa, identical to that of the core glycoprotein of rat N-syndecan.
Comparative localization of immunoreactivity for N-syndecan in the developing rat (16days) vomeronasal system.
Double immunofluorescence labelling with anti-NCAM (green) and anti-N-syndecan (red). N-syndecan immunoreactivity surrounds NCAM-immunoreactive vomeronasal axons and their associated cell clusters (asterisk).(Ref.2)
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