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Anti N-syndecan Polyclonal Antibody

Background

Syndecan is the family name of transmembrane proteoglycans that carry predominantly heparan sulfate side chains. This proteoglycan family consists of four members. N-syndecan (syndecan-3) is the principal member expressed during early postnatal development in both central and peripheral nervous systems. N-syndecan binds various heparin-binding growth factors such as FGFs via the heparan sulfate moiety, and communicates with the cytoskeleton via the cytoplasmic domain of the core protein. N-syndecan plays a pivotal role in formation of the neural network through these molecular interactions. This antibody recognizes effectively the core protein of N-syndecan.

Application

Western blotting



Characterization of the polyclonal anti-N-syndecan antiserum
Immunoblotting using anti-Nsyndecan of a partially purified preparation of membrane-bound proteoglycan (lane 1), and that digested with chondroitinase ABC (lane 2), and heparitinase I (lane 3). After the heparitinase I treatment , the immunoreactive band is sifted to an apparent molecular weight of approximately 140 kDa, identical to that of the core glycoprotein of rat N-syndecan.



Immunohistochemistry



Comparative localization of immunoreactivity for N-syndecan in the developing rat (16days) vomeronasal system.
Double immunofluorescence labelling with anti-NCAM (green) and anti-N-syndecan (red). N-syndecan immunoreactivity surrounds NCAM-immunoreactive vomeronasal axons and their associated cell clusters (asterisk).(Ref.2)

Product List

Product Name Cat# Quantity Price

Anti N-syndecan

CAC-NU-07-004 100UL

¥ 50,000
$ 667
€ 500

References
  • Watanabe, E., Matsui, F., Keino, H., Ono, K., Kushima, Y., Noda, H.. & Oohira, A., A membrane-bound heparan sulfate proteoglycan that is transiently expressed on growing axons in the rat brain, (1996) J. Neurosci. Res., 44, 84-96.
  • Toba, Y., Horie, M., Sango, K., Takashiki, A., Matsui, F., Oohira, A., & Kawano, H., Expression and immunohistochemical localization of heparan sulfate proteoglycan N-syndecan in the migratory pathway from the rat olfactory placode, (2002) Eur. J. Neurosci., 15, 1-13.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.