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Anti Neurocan peptides Polyclonal Antibody

Background

Neurocan is a nervous tissue-unique, secretory proteoglycan that carries predominantly chondroitin sulfate side chains. Its expression gradually decreases with the nervous tissue development. In the immature brain, neurocan exists in a full-length form with a 240 kDa-core glycoprotein, whereas it exists totally in proteolytic fragments of the NH2-terminal half (neurocan-N) with a 130 kDa-core glycopeptide and the COOH-terminal half (neurocan-C) with a 150 kDa-core glycopeptide. Neurocan is implicated in the neural network formation, and is a susceptibility factor for bipolar disorder. This proteoglycan is upregulated in the lesion site of the central nervous system, and is a major component of the glial scar. In addition, neurocan-N, not neurocan-C, is detectable in the perineuronal nets of some neurons. This antibody recognizes effectively neurocan-N as well as the full-length neurocan.

Specification

Host Rabbit
Specificity Neurocan core protein
Antigen A synthetic peptide, KGLNGRHFQQQGPEDQ, corresponding to the portion of amino
acids 483-498 that is contained in the NH2-terminal half of the rat neurocan core protein
Cross reactivity Rat, Mouse
Applications Western blotting: 1 / 200, Immunohistochemistry: 1 / 200 (Paraffin)

Application

Western blotting



Characterization of Neurocan peptides pAb and Neurocan peptides mAb (clone 1G2) with samples obtained from 10-day-old rat brain.
The PBS-soluble brain CSPG mixture of 10-day-old rats (lane 1) and the brain PBS-extract digested with chondroitinase ABC (lanes 2 and 3) were separated by SDS-PAGE. Neurocan peptides pAb recognized a very diffuse band with the same mobility as that recognized by Neurocan peptides mAb (clone 1G2). Monoclonal antibody (clone 1G2) recognized both 220 and 150 kDa core glycoproteins in the chondroitinase-digested sample. Polyclonal antibody recognized not only the 220 kDa core glycoprotein but also that with a molecular weight of 130 kDa in both. (Ref.3)



Immunohistochemistry



Distribution of neurocan in the adult rat cerebral cortex
A. The cell surfaces of some neurons were immunoreactive for anti-neurocan polyclonal antibody
B. Confocal image of the cerebral cortex of an adult rat double-labeled for neurocan-130 (red) and synaptophysin (green). Note neurocan-130 immunoreactivity surrounding
a neuronal cell body (n) and a proximal dendrite (large arrow). Neurocan-130 is absent in the axon terminals recognized by anti-synaptophysin (small arrows). (Ref.2)

Product List

Product Name Cat# Quantity Price

Anti Neurocan Peptides

CAC-NU-07-005 200UL

¥ 50,000
$ 667
€ 500

References
  • Oohira, A., Matsui, F., Tokita, Y., Yamauchi, S., & Aono, S., Molecular interactions of neural chondroitin sulfate proteoglycans in the brain development, (2000) Arch. Biochem. Biophys., 374, 24-34.
  • Fumiko Matsui, Masako Nishizuka, Yoko Yasuda, Sachiko Aono, Eiji Watanabe, Atsuhiko Oohira ., Occurrence of a N-terminal proteolytic fragment of neurocan, not a C-terminal half, in a perineuronal net in the adult rat cerebrum, (1998) Brain Res., 790, 45-51
  • Matsui, F., Watanabe, E., & Oohira, A., Immunological identification of two proteglycan fragments derived from neurocan, a brain-specific chondroitin sulfate proteoglycan, (1994) Neurochem. Int., 25, 425-431.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.