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Antibody for Histone H3.3

Anti Histone H3.3 Monoclonal Antibody (4H2D7)

Background

Nucleosomes are composed of four different histone proteins, designated H3, H4, H2A, and H2B. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in eukaryotes. Deposition of Histone H3.1 is coupled to DNA synthesis during DNA replication and possibly DNA repair.

Application

・ Western blotting: 1/1000
・ Immunocytochemistry: 1/2500
・ ChIP
・ IP

Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

CAC_CE040B_2
Western blot analysis of HeLa cell extracts using Histone H3.3 antibody,4H2D7 .
CAC_CE040B_3
Immunocytochemical analysis of HeLa Cell using Histone H3.3 antibody, 4H2D7.

The composition of Histone H3 variants peptides and the reactivity of Histone H3.3 antibody, 4H2D7.

CAC_CE040B_1

H3.1 prptide     
H3.3 prptide 
21   
21 
ATKAARKSAPATGGVKKPH
ATKAARKSAPSTGGVKKPH 
   39
   39 

Product List

Product Name Cat# Quantity Price

Anti Histone H3.3

CAC-CE-040B 50UL

¥ 58,000
$ 774
€ 580

References

1) Hake and Allis, (2006) PNAS, 103, 6428-6435
2) Harada et al., (2012) EMBO J. doi: 10.1038/emboj.2012.136.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.