Nucleosomes are composed of four different histone proteins, designated H3, H4, H2A, and H2B. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in eukaryotes. Histone H3.3 serves as the replacement variant for the DNA-synthesis-independent deposition pathway.
・ Western blotting: 1/10000
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.
Western blot analysis of recombinant protein and mammalian cell extracts using Histone H3.3 antibody, 6C4A3.
Anti Histone H3.3
1) Hake and Allis, (2006) PNAS, 103, 6428-6435
2) Harada et al., (2012) EMBO J. doi: 10.1038/emboj.2012.136.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.