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Antibody for Histone H3.1/H3.2

Anti Histone H3.1/H3.2 Monoclonal Antibody (1D4F2)

Background

Nucleosomes are composed of four different histone proteins, designated H3, H4, H2A, and H2B. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in eukaryotes. Deposition of Histone H3.1 is coupled to DNA synthesis during DNA replication and possibly DNA repair.

Application

・ Western blotting: 1/2000
・ Immunocytochemistry: 1/2500
・ ChIP
・ IP

Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Western blot analysis of HeLa cell extracts using Histone H3.1/H3.2 antibody, 1D4F2.
Immunocytochemical analysis of HeLa Cell using Histone H3.1/H3.2 antibody, 1D4F2.

The composition of Histone H3 variants peptides and the reactivity of Histone H3.1/H3.2 antibody, 1D4F2.

H3.1 peptide     
H3.3 peptide
21  
21 
ATKAARKSAPATGGVKKPH
ATKAARKSAPSTGGVKKPH
  39
  39 

Product List

Product Name Cat# Quantity Price

Anti Histone H3.1/H3.2

CAC-CE-039B 50UL

¥ 58,000
$ 774
€ 580

References

1) Hake and Allis, (2006) PNAS, 103, 6428-6435

2) Harada et al., (2012) EMBO J. doi: 10.1038/emboj.2012.136.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.