Nucleosomes are composed of four different histone proteins, designated H3, H4, H2A, and H2B. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in eukaryotes. Deposition of Histone H3.1 is coupled to DNA synthesis during DNA replication and possibly DNA repair.
・ Western blotting: 1/2000
・ Immunocytochemistry: 1/2500
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.
|Western blot analysis of HeLa cell extracts using Histone H3.1/H3.2 antibody, 1D4F2.|
|Immunocytochemical analysis of HeLa Cell using Histone H3.1/H3.2 antibody, 1D4F2.|
Anti Histone H3.1/H3.2
1) Hake and Allis, (2006) PNAS, 103, 6428-6435
2) Harada et al., (2012) EMBO J. doi: 10.1038/emboj.2012.136.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.