Post-translation modifications of histones modulate the accessibility and transcriptional competence of specific chromatin regions within the eukaryotic genome. Phosphorylation of histone H3 is unique in the sense that it associates on one hand with open chromatin during gene activation and marks on the other hand highly condensed chromatin during mitosis.
・ Western blotting: 1/500
・ Immunocytochemistry: 1/500
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.
|M: Add Nozodazole.
I: After 12 hours from released Nocodazole.
(Whole cell extracts)
|-: Before UV irradiation
+: After UV irradiation (100J/m2, 2 hours)
Western blot analysis of the treated-cell extracts using Histone H3 T32ph antibody, 6C7G12.
|Immunocytochemical analysis of eLa Cell using Histone H3 T32ph antibody, 6C7G12.
(left : interphase, right : metaphase).
|21 ATKAARKS APAT GGVKKPH 39
21 ATKAARKSphAPAT GGVKKPH 39
21 ATKAARKS APATphGGVKKPH 39
21 ATKAARKS APST GGVKKPH 39
21 ATKAARKSphAPST GGVKKPH 39
21 ATKAARKS APSTphGGVKKPH 39
1 ARTKQTARKSTphGGKAPRKQC 19
Anti Histone H3 T32ph
1) Strahl and Allis, (2000) Nature 403, 41-45.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.