Post-translation modifications of histones modulate the accessibility and transcriptional competence of specific chromatin regions within the eukaryotic genome. Phosphorylation of histone H3 Threonine 11 (H3-T11ph) occurs throughout the cell cycle and is Chk1 dependent. It has reported that DNA damage rapidly reduces H3-T11 phosphorylation.
・ Western blotting: 1/500
・ Immunocytochemistry: 1/500
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.
|M: Add Nocodazole.
I: After 12 hours from released Nocodazole.
(Whole cell extracts)
|-: Before UV irradiation
+: After UV irradiation (100J/m2, 2 hours)
After released G2/M synchronization.
tsFT210...Cdk1 mutant strain
Western blot analysis of the treated-cell extracts using Histone H3 T11ph antibody, 6G12C5.
|Immunocytochemical analysis of HeLa Cell using Histone H3 T11ph antibody, 6G12C5
(left : interphase, right : metaphase).
|H3 1 ARTKQTARK S T GGKAPRKQC 19
H3 K9ac 1 ARTKQTARKac S T GGKAPRKQC 19
H3 S10ph 1 ARTKQTARK Sph T GGKAPRKQC 19
H3 T11ph 1 ARTKQTARK S TphGGKAPRKQC 19
H3 K9acT11ph 1 ARTKQTARKac S TphGGKAPRKQC 19
H3 S10phT11ph 1 ARTKQTARK Sph TphGGKAPRKQC 19
H3.1 T32ph 21 ATKAARKSAPA TphGGVKKPH 39
H3.3 T32ph 21 ATKAARKSAPS TphGGVKKPH 39
Anti Histone H3 T11ph
1) Shimada et al., (2008) Cell 132, 221?232.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.