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Antibody for modification of histone H3

Anti Histone H3 T11ph Monoclonal Antibody

Background

Post-translation modifications of histones modulate the accessibility and transcriptional competence of specific chromatin regions within the eukaryotic genome. Phosphorylation of histone H3 Threonine 11 (H3-T11ph) occurs throughout the cell cycle and is Chk1 dependent. It has reported that DNA damage rapidly reduces H3-T11 phosphorylation.

Application

・ Western blotting: 1/500
・ Immunocytochemistry: 1/500

Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

CAC_CE035A_2 M: Add Nocodazole.
I: After 12 hours from released Nocodazole.
(Whole cell extracts) 
CAC_CE035A_3 -: Before UV irradiation
+: After UV irradiation (100J/m2, 2 hours)
(Chromatin fractions) 
CAC_CE035A_4

After released G2/M synchronization.
(Whole cell extracts)

tsFT210...Cdk1 mutant strain 

Western blot analysis of the treated-cell extracts using Histone H3 T11ph antibody, 6G12C5.

CAC_CE035A_5
Immunocytochemical analysis of HeLa Cell using Histone H3 T11ph antibody, 6G12C5
(left : interphase, right : metaphase).

The composition of Histone H3 peptides and the reactivity of Histone H3 T11ph antibody, 6G12C5.

CAC_CE035A_1
H3                      1  ARTKQTARK    S     T   GGKAPRKQC   19
H3 K9ac               1  ARTKQTARKac S     T   GGKAPRKQC   19
H3 S10ph             1  ARTKQTARK    Sph T   GGKAPRKQC   19
H3 T11ph             1  ARTKQTARK    S     TphGGKAPRKQC   19
H3 K9acT11ph       1  ARTKQTARKac S     TphGGKAPRKQC   19
H3 S10phT11ph     1  ARTKQTARK    Sph TphGGKAPRKQC   19
H3.1 T32ph           21   ATKAARKSAPA     TphGGVKKPH       39
H3.3 T32ph           21   ATKAARKSAPS     TphGGVKKPH       39 

Product List

Product Name Cat# Quantity Price

Anti Histone H3 T11ph

CAC-CE-035A 100UL

¥ 58,000
$ 774
€ 580

References

1) Shimada et al., (2008) Cell 132, 221?232.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.