Post-translation modifications of histones modulate the accessibility and transcriptional competence of specific chromatin regions within the eukaryotic genome. Phosphorylation of histone H3 is unique in the sense that it associates on one hand with open chromatin during gene activation and marks on the other hand highly condensed chromatin during mitosis.
・ Western blotting: 1/2500
・ Immunocytochemistry: 1/5000
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.
|Western blot analysis of HeLa whole cell extracts using Histone H3 S10ph antibody, 6G8B7.|
|Immunocytochemical analysis of HeLa Cell using Histone H3 S10ph antibody, 6G8B7.|
|H3 peptide 1 ARTKQTARKS TGGKAPRKQ 19
H3 S10ph peptide 1 ARTKQTARKSphTGGKAPRKQ 19
H3 S28ph peptide 21 ATKAARKSphAPATGGVKKPH 39
Anti Histone H3 S10ph
1) Strahl and Allis, (2000) Nature 403, 41-45.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.