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Anti Histone H3 K9Ac Monoclonal Antibody

Background

Post-translation modifications of histones modulate the accessibility and transcriptional competence of specific chromatin regions within the eukaryotic genome. Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly.

Application

・ Western blotting: 1/1000
・ Immunocytochemistry: 1/1000

Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

CAC_CE037A_3 Western blot analysis of HeLa whole cell extracts using Histone H3 K9ac antibody, 2G1F9.
CAC_CE037A_4
Immunocytochemical analysis of HeLa Cell using Histone H3 K9ac antibody, 2G1F9.

 

The composition of Histone H3 peptides and the reactivity of Histone H3 K9Ac antibody, 2G1F9

CAC_CE037A_1
H3 peptide                     1 ARTKQTARK  ST  GGKAPRKQ   19 
H3 K9ac peptide             1 ARTKQTARKacST  GGKAPRKQ  19 
H3 K9acT11ph peptide    1 ARTKQTARKacSTphGGKAPRKQ 19
CAC_CE037A_2
H3 K9ac peptide           1  ARTKQTARKacSTGGKAPRKQ    19
H3 K27ac peptide         21   AATKAARKacSAPATGGVKKPH 39

Product List

Product Name Cat# Quantity Price

Anti Histone H3 K9Ac

CAC-CE-037A 100UL

¥ 58,000
$ 774
€ 580

References

1) Strahl and Allis, (2000) Nature 403, 41-45.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.