Fibromodulin, encoded by the FMOD gene, is a member of a family of small interstitial proteoglycans containing a central region composed of leucine-rich repeats with 4 keratan sulfate chains flanked by disulfide-bonded terminal domains. Its core protein is roughly 58 kDa in size and in its fully glycosylated form reaches 150-200 kDa in molecular weight. Fibromodulin has been proposed to participate in the assembly of the extracellular matrix by linking to collagen type I and II and (negatively) controlling their fibrillogenesis in vitro and in vivo (as also confirmed by the altered collagen fibril structure observed in FMOD null mice). Fibromodulin may also influence TGF signalling by sequestering latent TGF-β(s) into the extracellular matrix and is recognized to be a primary component of the tumour stroma (particularly well documented in epithelial tumours). Recent observations suggest that fibromodulin is a primary prognostic indicator in chronic lymphocytic B-cell leukemia.
Recommended use : WB, IHC
Western blotting : 1/30 1/60
Immunohistochemistry, 1/25 to 1/75
Staining Pattern : Tendon, cartilages, various stromal tissues, various tumour cells and tumour stroma
(A) Immunohistochemical staining of human articular cartilage;.
(B) Western blotting of undigested and endo-β-galactosidase-digested human cartilage fibromodulin resolved by SDS-PAGE on 7% gels under reducing conditions (band at 60 kDa presumably corresponds to unglycosylated fibromodulin molecules of the intracellular pool).
(C) Immunohistochemical staining of a leiomyosarcoma lesion (labelling is primarily concentrated in the stromal compartment);
Anti Fibromodulin (636B12)
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.