The RhoGAP family embraces a unique member named XTP1 (also referred to as DEPDC1B, BRCC3 or FLJ11252) and pairing with a homologue denoted SDP35 (also referred to as DEPDC1, DEP8, FLJ20354 or DEPDC1-V2). The structural-functional properties of XTP1 is still largely unknown, but its structural uniqueness resides in the presence of a domain showing homology with Dishwelled, i.e. the DEP domain (Dishwelled/Pleckstrin-like domain). The presence of this domain suggests that XTP1 might engage in more complex molecular interactions than those involving other members of the family. Another peculiar feature of the RhoGAP is represented by its atypical GAP domain, which lacks the orthodox “Arg finger” catalytic motif essential for exerting a canonical GAP function. Whereas most RhoGAP family members are either ubiquitously expressed throughout the body, or are concentrated in discrete tissue/organs, XTP1 is overall remarkably poorly represented in most human adult tissues (as also evidenced by information available through the Comparative Cancer Genome Project database). XTP1 is de novo expressed upon neoplastic transformation and remains abundant in many cancer cell lines. Some observations in epithelial tumours suggest that it may act as a cell-cycle regulator.
Recommended use : WB, IP, IHC
Western blotting : 1/30 1/60, Band at 60 kDa
Immunohistochemistry, 1/25 to 1/75
Anti XTP1 (2191H1)
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.