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Anti Plectin antibody

Specification

Clone: PC742
Host Mouse
Specificity C-terminal region of plectin
Antigen An expressed recombinant His-tagged fusion protein of human Plectin
(U53204, 2,930 - 3,153aa).
Clone PC742
Subclass IgG1
Cross reactivity Human, mouse, rabbit. pig, bovine
Applications WB, IF, IP (Other applications have not been tested.)
Clone: PN753
Host Mouse
Specificity N-terminal region of plectin
Antigen An expressed recombinant His-tagged fusion protein of human plectin
(U53204, 173 - 595aa)
Clone PN753
Subclass IgG1
Cross reactivity Human, rat, rabbit. pig
Applications WB, IF, IP (Other applications have not been tested.)

Example Assay Data

Western blot analysis



Fig.1 Western blot analysis
Whole cell lysaes prepared from DJM-1 cells (lane 1) and HeLa cells (lane 2)
were immunoblotted with PN753 or PC742 at 1:200 dilution.
Plectin antibodies detected approximate 500 kDa bands in these cell lysates (arrow).
Smaller polypeptide found in lane 2 may be a degraded product or alternatively spliced rod-less isoform of plectin.
Polypeptiedes were separated by SDS-PAGE (5% separating gel).


Location of the epitopes for the plectin antibodies



Fig.2 Location of the epitopes for the plectin antibodies
PN753 and PC742 clones were obtained by immunizing
mice with the NH2- (173-595aa) or the COOH-terminal
(2,930-3,153aa) regions of human plectin (4,574aa),
respectively. Gray box represents a predicted coiled-coil
region (1,300-2,600aa).


Immunofluorescence microscopy of human skin



Fig.3 Immunofluorescence microscopy of human skin
Human skin sections were stained with PC742 (1:100 dilution) or PN753 (1:100 dilution).
Arrows indicate dermal-epidermal junctions. PN753 stains epidermal cells
in addition to hemidesmosomes at the dermal-epidermal junction.
Sections were fixed with -20℃ acetone for 10 min. Bar: 50um.

Product List

Product Name Cat# Quantity Price

Anti plectin

CAC-NU-01-PLC 500UL

¥ 40,000
$ 534
€ 400

Anti plectin

CAC-NU-01-PLN 500UL

¥ 40,000
$ 534
€ 400

References
  • Hirako Y, Yonemoto Y, Yamauchi T, Nishizawa Y, Kawamoto Y, Owaribe K.
    Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line.
    Exp. Cell Res., 324:172-182, 2014

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.