Follistatin also known as activin-binding protein is a protein that in humans is encoded by the FST gene. Follistatin is an autocrine glycoprotein that is expressed in nearly all tissues of higher animals. It was initially isolated from follicular fluid and was identified as a protein fraction that inhibited follicle-stimulating hormone (FSH) secretion from the anterior pituitary, and so was known as FSH-suppressing protein (FSP). Since then its primary function has been determined to be the binding and bioneutralization agent of members of the TGF-β superfamily, with primary focus on activin.
IHC, RIA, Western blotting, Neutralization
RIA : 1/2,000 - 1/10,000
IHC : 1/200 - 1/1,000
WB : 1/200 - 1/1,000
Elution profile on reverse-phase HPLC of the partially purified activin-follistain complex of fraction 2 applied to a Cosmosil 5C18-AR column (10*250 mm). The column was developed with the indicated gradient of acetonitrile (ACN) in 0.1 % tri-fluoroacetic acid.
< Reference >
Hasegawa Y, Eto Y, Ibuki Y & Sugino H (1994) Activin as autocrine and paracrine factor in the ovary. Horm Res 41(Suppl 1): 55-62
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.