Forkhead transcription factor FoxO controls various cellular processes involved in cell cycle, cell death, metabolism and oxidative stress. Recent studies have also suggested FoxO is a key molecule for lifespan regulation of various organisms. Drosophila has a single FoxO gene (dFoxO), inactivation of which is dispensable for survival. However, dFoxO appears to regulate resistance to oxidative stress and aging in Drosophila. The activity of dFoxO is regulated by phosphorylation, ubiquitination and acetylation, which may cause multiple bands of dFoxO in western blotting using this antibody. This antibody detects endogenous levels of total dFoxO protein.
Expression of dFoxO in the fly brain lysates was detected with anti dFoxO (1:3000 dilution) combined with the ECL Plus reagent.
Endogenous dFoxO from S2 cells was Immunoprecipitated with anti dFoxO.
1) Kanao T, et al. Activation of FoxO by LRRK2 induces expression of proapoptotic proteins and alters survival of postmitotic dopaminergic neuron in Drosophila. Hum Mol Genet. 19, 3747-3758, 2010
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