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useful for the research of a role in apoptosis, mitochondrial morphology and autophagy

Anti BIF1 (Clone: BIF1-443) monoclonal antibody

Background

Bif1 (Bax interacting factor 1; also known as SH3GLB1 was initially cloned as a binding partner of the pro-apoptotic Bax protein. This protein is a member of the endophilin B protein family and contains an amino-terminal N-BAR domain and a carboxy-terminal Src homology 3 (SH3) domain1), . Bif1 gene encodes a predicted protein of 365 amino acid and mouse and human Bif-1 protein are highly conserved and share 96% identity at the amino acids level. RNA expression of Bif-1 in human tissue is abundant in heart, skeletal muscle, kidney, and placenta1). Bif-1 protein plays a critical role in apoptosis, mitochondrial morphology and autophagy. Loss of Bif-1 suppresses programmed cell death and promotes tumorigenesis5).

Application

< Western blot >
Anti BIF1 antibody
Wild type (left lane) or Bif-1 deficient (right lane) mouse embryonic fibroblast with anti Bif-1 antibody (clone #BIF1-443) at 1:500 dilution blocked with 3% skim milk in TBS-T. Predicted band size is around 42 kDa (calculated weight is 40.8 kDa according to NP_062337.1).

Applying Campaign Price (from July 1st - November 30th, 2017)

⇒ Autophagy antibody products 30% discount campaign

Product List

Product Name Cat# Quantity Price

Anti BIF1

CAC-CTB-BF-M01-W 50UG

¥ 35,000
$ 667
€ 500

References
  • 1. Cuddeback SM, Yamaguchi H, Komatsu K, Miyashita T, Yamada M, Wu C, Singh S, Wang HG: Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax. J Biol Chem. 2001: 276(23):20559-65. PMID: 11259440
  • 2. Pierrat B, Simonen M, Cueto M, Mestan J, Ferrigno P, Heim J: SH3GLB, a new endophilin-related protein family featuring an SH3 domain. Genomics. 2001: 71(2): 222-34. PMID: 11161816
  • 3. Karbowski M, Jeong SY, Youle RJ. Endophilin B1 is required for the maintenance of mitochondrial morphology. J Cell Biol. 2004:166: 102739. PMID: 15452144
  • 4. Takahashi Y, Meyerkord CL, Wang HG: Bif-1/endophilin B1: a candidate for crescent driving force in autophagy. Cell Death Differ. 2009: 16(7): 947-55. PMID: 19265852
  • 5. Takahashi Y, Coppola D, Matsushita N, Cualing HD, Sun M, Sato Y, Liang C, Jung JU, Cheng JQ, Mul JJ, Pledger WJ, Wang HG: Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis. Nat Cell Biol. 2007: 9(10): 1142-51. PMID: 17891140

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.