Bif1 (Bax interacting factor 1; also known as SH3GLB1 was initially cloned as a binding partner of the pro-apoptotic Bax protein. This protein is a member of the endophilin B protein family and contains an amino-terminal N-BAR domain and a carboxy-terminal Src homology 3 (SH3) domain1), . Bif1 gene encodes a predicted protein of 365 amino acid and mouse and human Bif-1 protein are highly conserved and share 96% identity at the amino acids level. RNA expression of Bif-1 in human tissue is abundant in heart, skeletal muscle, kidney, and placenta1). Bif-1 protein plays a critical role in apoptosis, mitochondrial morphology and autophagy. Loss of Bif-1 suppresses programmed cell death and promotes tumorigenesis5).
< Western blot >
Wild type (left lane) or Bif-1 deficient (right lane) mouse embryonic fibroblast with anti Bif-1 antibody (clone #BIF1-443) at 1:500 dilution blocked with 3% skim milk in TBS-T. Predicted band size is around 42 kDa (calculated weight is 40.8 kDa according to NP_062337.1).
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.