Register now!Newsletter subscription

  • CATALOG PDF Download
  • BROCHURES PDF Download

New oxidative stress marker

Anti Nitroguanosine monoclonal antibody

   Oxidative stress marker product TOP


8-Nitroguanosine is a nitrated nucleic acid which is formed by peroxynitrite, myeloperoxidase, nitrite, and peroxide. It is known that the nitration of guanine is enhanced in virus infection1, 2, bacterial infection3, 4, inflammatory disease5, cancers5, and diseases associated with smoking6. 8-nitroguanosine is thought to be one of the makers of DNA damage caused by oxidative stress. Cyclic GMP (cGMP) is one of the important substances for the signal transfer. On the other hand, 8-Nitro-cGMP (nitrated cGMP) has been identified in vivo3. Therefore, 8-Nitro-cGMP can potentially act as a mediator for reactive oxygen signaling3, 7-9. Although the product, Anti-Nitroguanosine monoclonal antibody (NO2G52), does not cross-react with normal nucleotide bases, it selectively reacts with nitro functionalized nucleotides such as nitro-cGMP, nitro-GMP, and nitro-GTP. Therefore, Anti-Nitroguanosine polyclonal antibody is universal antibody of nitrated guanine which modified 8th position of guanine with nitro group. NO2G52 is commonly used for immunostaining. In addition, NO2G52 can potentially utilized for affinity purification of nitroguanine derivative3,6.


Immunohistochemistry, 10 ug/ml
ELISA, 1 ug/ml 

[Production of 8-nitroguanine by airway epithelial cell with ldiopathic Fibrosis (IPS)]

A. 8-nitroguanine (green) and iNOS (red) 
B. Bright-field 
C. 8-nitroguanine (green) and 8-oxoguanine (Red)
D. 8-nitroguanine (green) and mitochondria (red)


[Endogenous guanine nitration in RAW 264.7 cells, a murine macrophage cell line]

Product List

Product Name Cat# Quantity Price

Anti Nitroguanosine


¥ 40,000
$ 534
€ 400

  • Cytoprotective role of the fatty acid binding protein 4 against oxidative and endoplasmic reticulum stress in 3T3-L1 adipocytes.
    Kajimoto K, Minami Y, Harashima H.
    FEBS Open Bio. 2014 Jul 3;4:602-10. PMID: 25161868
  • Photoprotection by 1(alpha),25-Dihydroxyvitamin D3 and vitamin D-like compound in human skin
    Song, Eric Jung Yong
    A Thesis Submitted for the Degree of Master of Philosophy (Medicine) The University of Sydney PMID:
  • 1α,25 dihydroxyvitamin D3 enhances cellular defences against UV-induced oxidative and other forms of DNA damage in skin.
    Gordon-Thomson C, Gupta R, Tongkao-on W, Ryan A, Halliday GM, Mason RS.
    Photochem Photobiol Sci. 2012 Dec;11(12):1837-47. PMID: 23069805

  • Protein cysteine S-guanylation and electrophilic signal transduction by endogenous nitro-nucleotides.
    Ahmed KA, Sawa T, Akaike T
    Amino Acids. 2011 Jun;41(1):123-30. PMID: 20213439
  • Regulation of redox signaling involving chemical conjugation of protein thiols by nitric oxide and electrophiles.
    Sawa T, Arimoto H, Akaike T.
    Bioconjug Chem. 2010 Jul 21;21(7):1121-9. PMID: 20225829
  • Cytoprotective function of heme oxygenase 1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis.
    Zaki MH, Fujii S, Okamoto T, Islam S, Khan S, Ahmed KA, Sawa T, Akaike T.
    J Immunol. 2009 Mar 15;182(6):3746-56. PMID: 19265153
  • Nitrated cyclic GMP as a new cellular signal.
    Feelisch M.
    Nat Chem Biol. 2007 Nov;3(11):687-8. PMID: 17948012
  • Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate.
    Sawa T, Zaki MH, Okamoto T, Akuta T, Tokutomi Y, Kim-Mitsuyama S, Ihara H, Kobayashi A, Yamamoto M, Fujii S, Arimoto H, Akaike T.
    Nat Chem Biol. 2007 Nov;3(11):727-35. PMID: 17906641
  • Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification: association with cigarette smoking.
    Sawa T, Tatemichi M, Akaike T, Barbin A, Ohshima H.
    Free Radic Biol Med. 2006 Feb 15;40(4):711-20. PMID: 16458202
  • Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis.
    Terasaki Y, Akuta T, Terasaki M, Sawa T, Mori T, Okamoto T, Ozaki M, Takeya M, Akaike T.
    Am J Respir Crit Care Med. 2006 Sep 15;174(6):665-73. PMID: 16741153
  • Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity.
    Yoshitake J, Akaike T, Akuta T, Tamura F, Ogura T, Esumi H, Maeda H.
    J Virol. 2004 Aug;78(16):8709-19. PMID: 15280479
  • 8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis.
    Akaike T, Okamoto S, Sawa T, Yoshitake J, Tamura F, Ichimori K, Miyazaki K, Sasamoto K, Maeda H.
    Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):685-90. PMID: 12522148