8-Nitroguanosine is a nitrated nucleic acid which is formed by peroxynitrite, myeloperoxidase, nitrite, and peroxide. It is known that the nitration of guanine is enhanced in virus infection1, 2, bacterial infection3, 4, inflammatory disease5, cancers5, and diseases associated with smoking6. 8-nitroguanosine is thought to be one of the makers of DNA damage caused by oxidative stress. Cyclic GMP (cGMP) is one of the important substances for the signal transfer. On the other hand, 8-Nitro-cGMP (nitrated cGMP) has been identified in vivo3. Therefore, 8-Nitro-cGMP can potentially act as a mediator for reactive oxygen signaling3, 7. Anti-Nitroguanosine polyclonal antibody, does not cross-react with normal nucleobases, it selectively reacts with nitrated nucleic acid such as nitroguanosine, nitroguanine, and nitroxanthine. Therefore, Anti-Nitroguanosine polyclonal antibody is universal antibody of nitrated guanine which modified 8th position of guanine with nitro group. Anti-Nitroguanosine polyclonal antibody has very high affinity for 8-nitroguanine and 8-nitroguanosine, but it does not cross-react with normal guanosine, guanine, 8-hydroxyguanine or 3-nitrotyrosine. Since this antibody was prepared using rabbits, it can be used for immuno-histostaining of rodent tissues.
Immunohistochemistry, 10 ug/ml
ELISA, 10 ug/ml
[The Reactivity of Aniti-Nitroguanosine polyclonal antibody]
[Immunostaining example of influenza virus-infected mouse lung epithelial cell]