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New oxidative stress marker

Anti Nitroguanosine polyclonal antibody

   Oxidative stress marker product TOP

Background

8-Nitroguanosine is a nitrated nucleic acid which is formed by peroxynitrite, myeloperoxidase, nitrite, and peroxide. It is known that the nitration of guanine is enhanced in virus infection1, 2, bacterial infection3, 4, inflammatory disease5, cancers5, and diseases associated with smoking6. 8-nitroguanosine is thought to be one of the makers of DNA damage caused by oxidative stress. Cyclic GMP (cGMP) is one of the important substances for the signal transfer. On the other hand, 8-Nitro-cGMP (nitrated cGMP) has been identified in vivo3. Therefore, 8-Nitro-cGMP can potentially act as a mediator for reactive oxygen signaling3, 7. Anti-Nitroguanosine polyclonal antibody, does not cross-react with normal nucleobases, it selectively reacts with nitrated nucleic acid such as nitroguanosine, nitroguanine, and nitroxanthine. Therefore, Anti-Nitroguanosine polyclonal antibody is universal antibody of nitrated guanine which modified 8th position of guanine with nitro group. Anti-Nitroguanosine polyclonal antibody has very high affinity for 8-nitroguanine and 8-nitroguanosine, but it does not cross-react with normal guanosine, guanine, 8-hydroxyguanine or 3-nitrotyrosine. Since this antibody was prepared using rabbits, it can be used for immuno-histostaining of rodent tissues.

Application

Immunohistochemistry, 10 ug/ml
ELISA, 10 ug/ml

 

[The Reactivity of Aniti-Nitroguanosine polyclonal antibody]

[Immunostaining example of influenza virus-infected mouse lung epithelial cell]

Product List

Product Name Cat# Quantity Price

Anti Nitroguanosine

CAC-KMU-P01 200UG

¥ 37,000
$ 494
€ 370

Citation
  • Inhibition of intestinal polyp formation by pitavastatin, a HMG-CoA reductase inhibitor.
    Teraoka N, Mutoh M, Takasu S, Ueno T, Yamamoto M, Sugimura T, Wakabayashi K.
    Cancer Prev Res (Phila). 2011 Mar;4(3):445-53. PMID: 21233289

References
  • Protein cysteine S-guanylation and electrophilic signal transduction by endogenous nitro-nucleotides.
    Ahmed KA, Sawa T, Akaike T.
    Amino Acids. 2011 Jun;41(1):123-30. PMID: 20213439
  • Regulation of redox signaling involving chemical conjugation of protein thiols by nitric oxide and electrophiles.
    Sawa T, Arimoto H, Akaike T.
    Bioconjug Chem. 2010 Jul 21;21(7):1121-9. PMID: 20225829
  • Cytoprotective function of heme oxygenase 1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis.
    Zaki MH, Fujii S, Okamoto T, Islam S, Khan S, Ahmed KA, Sawa T, Akaike T.
    J Immunol. 2009 Mar 15;182(6):3746-56. PMID: 19265153
  • Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate.
    Sawa T, Zaki MH, Okamoto T, Akuta T, Tokutomi Y, Kim-Mitsuyama S, Ihara H, Kobayashi A, Yamamoto M, Fujii S, Arimoto H, Akaike T.
    Nat Chem Biol. 2007 Nov;3(11):727-35. PMID: 17906641
  • Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification: association with cigarette smoking.
    Sawa T, Tatemichi M, Akaike T, Barbin A, Ohshima H.
    Free Radic Biol Med. 2006 Feb 15;40(4):711-20. PMID: 16458202
  • Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis.
    Terasaki Y, Akuta T, Terasaki M, Sawa T, Mori T, Okamoto T, Ozaki M, Takeya M, Akaike T.
    Am J Respir Crit Care Med. 2006 Sep 15;174(6):665-73. PMID: 16741153
  • Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity.
    Yoshitake J, Akaike T, Akuta T, Tamura F, Ogura T, Esumi H, Maeda H.
    J Virol. 2004 Aug;78(16):8709-19. PMID: 15280479
  • 8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis.
    Akaike T, Okamoto S, Sawa T, Yoshitake J, Tamura F, Ichimori K, Miyazaki K, Sasamoto K, Maeda H.
    Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):685-90. PMID: 12522148