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BINKIT (R) for NK cells expansion from PBMCs

Feature and Advantages

・Natural killer (NK) cells can be expanded from PBMCs without feeder cells.
・The number of NK cells will increase several hundred to several thousand-fold in 3 weeks. NK cells will gain strikingly enhanced cytotoxic activity.
・This kit is suitable for PBMCs from 20 - 50ml of whole blood.
・Xeno-free (free from animal-derived materials) ・For research use only

Kit component

Contents

・NK Cell Initial Flask (75 cm2):1 flask
・NK Cell Initial Medium:45 ml
・NK Cell Initial Cocktail:1.9 ml
・NK Cell Subculture Medium:1000 ml

Options
・Culture bag(small, large)
・Culture bag connection tube

Example 1, vivo NK cell expansion using BINKIT(R)

NK cells were expanded from peripheral blood mononuclear cells (PBMCs) using BINKIT®. CD3-CD56+ cells were enriched from 15.8% to 88.6%.

Example 2, vivo NK cell expansion using BINKIT(R)

 

NK cells were expanded using BINKIT® from peripheral blood mononuclear cells (PBMCs) of healthy donors (HD; n=3). Cell count (left) and fold changes (right) indicate the efficient ex vivo expansion of NK cells.

Example 3, vivo NK cell expansion using BINKIT(R)

 
NK cell cytotoxicity against K562cell line was measured for BINKIT-expanded NK cells (solid lines) and freshly isolated NK cells (dashed lines) from three independent donors. The expanded NK cells exhibited significantly enhanced cytotoxicity against K562 cell line compared with the freshly isolated NK cells (P < 0.01). ;

Product List

Product Name Cat# Quantity Price

BINKIT for NK cells expansion from PBMCs

BIJ-N501-1 1KIT

¥ 176,000
$ 2347
€ 1760

BINKIT for NK cells expansion from PBMCs

BIJ-N501-2 2KIT Contact Us

BINKIT for NK cells expansion from PBMCs

BIJ-N501-4 4KIT Contact Us

BINKIT for NK cells expansion from PBMCs

BIJ-N501-8 8KIT Contact Us

NK cells

BIJ-FN100-1 1VIAL

¥ 30,000
$ 400
€ 300

References

◇Xuewen Deng et al ., Synergistic
cytotoxicity of ex vivo expanded natural killer cells in combination with monoclonal antibody
drugs against cancer cells, Int Immunopharmacol 14 (2012) 593-605

◇Xuewen Deng et al ., Ex vivo expanded natural killer cells can
possibly kill cancer stem cells, 18th ISCT Annual Meeting 2012

◇Xuewen Deng et al ., Cytotoxicity of expanded NK cells against
cancer cells is enhanced by monoclonal antibody drugs, 19th ISCT Annual Meeting 2013

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.