Tubulin is the major constituent of microtubules. There are three members (α, β, γ) and two subtypes (δ, ε) in the tubulin family. Of these members, beta tubulin (449 aa, 51 kDa) is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly during cell cycle.
• Western blotting: 1,000 fold dilution
• Immunofluorescent staining: assay dependent
Optimal dilutions/concentrations should be determined by the end user.
Western blot analysis of β-tubulin in the whole cell extracts of S. pombe (10 ug). Ant β-tublin antibody: 1,000 fold dilution
S.pombe β-tublin C-terminal peptide, C-YEIEEEKEPLEY-OH
Shipped at 4°C or -20°C. Upon arrival aliquot and store at -20°C.
1) Fedyanina, O.S.,et al “Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin-folding cofactor D in fission yeast cells." Yeast 26,: 235-247(2009) DOI:10.1002/yea.1663 (WB)
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.