Bacteriophage T4 derived DNA ligase catalyzes the formation of phosphodiester bonds between 3’-OH termini and 5’-P termini in duplex DNA or RNA (1). This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. T4 DNA ligase was expressed in E.coli in large quantities and highly purified. MW is 55.3 kDa.
1) Insertion of DNA fragment into a vector
2) Linker (or Adaptor) ligation with DNA fragment
|Concentration:||400 U/ul, where one unit is the amount of enzyme that ligates more than 90% of 6 ug of λDNA-HindIII fragments in a 20μl mixture in 30 minutes at 16°C.|
|Form:||10mM Tris-HCl (pH 7.6), 50mM KCl, 0.1mM EDTA, 1mM dithiothreitol, 50% glycerol|
|Quality Assurance:||Greater than 95% protein determined by SDS-PAGE (CBB staining) The absence of endonucleases and exonucleases was confirmed.|
|Reagents Supplied with Enzyme:||10 x T4 Ligase Reaction Buffer (T4-Lig): 500mM Tris-HCl (pH 7.6), 100mM MgCl2, 10 mM ATP, 100mM dithiothreitol|
|Data Link:||UniProtKB/Swiss-Prot P00970 (DNLI_BPT4)|
|Storage:||Store at -20°C|
T4 DNA Ligase
T4 DNA Ligase
1. Weiss B et al (1968) “Enzymatic breakage and joining of deoxyribonucleic acid.” J. Biol. Chem. 243:
4543-4555 PMID: 4879167
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.