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T4 DNA Ligase

Background

Bacteriophage T4 derived DNA ligase catalyzes the formation of phosphodiester bonds between 3’-OH termini and 5’-P termini in duplex DNA or RNA (1). This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. T4 DNA ligase was expressed in E.coli in large quantities and highly purified. MW is 55.3 kDa.

Application

1) Insertion of DNA fragment into a vector
2) Linker (or Adaptor) ligation with DNA fragment

Concentration: 400 U/ul, where one unit is the amount of enzyme that ligates more than 90% of 6 ug of λDNA-HindIII fragments in a 20μl mixture in 30 minutes at 16°C.
Form: 10mM Tris-HCl (pH 7.6), 50mM KCl, 0.1mM EDTA, 1mM dithiothreitol, 50% glycerol
Quality Assurance: Greater than 95% protein determined by SDS-PAGE (CBB staining) The absence of endonucleases and exonucleases was confirmed.
Reagents Supplied with Enzyme: 10 x T4 Ligase Reaction Buffer (T4-Lig): 500mM Tris-HCl (pH 7.6), 100mM MgCl2, 10 mM ATP, 100mM dithiothreitol
Data Link: UniProtKB/Swiss-Prot P00970 (DNLI_BPT4)
Storage: Store at -20°C

Product List

Product Name Cat# Quantity Price

T4 DNA Ligase

BAM-02-050-EX 20000UNIT

¥ 3,500
$ 47
€ 35

T4 DNA Ligase

BAM-02-050-5EX 5*20000UNIT

¥ 13,000
$ 174
€ 130

References

1. Weiss B et al (1968) “Enzymatic breakage and joining of deoxyribonucleic acid.” J. Biol. Chem. 243:
4543-4555 PMID: 4879167

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.