Schizosaccharomyces pombe Pad1, a 35 kDa protein, is a component of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Transcription factor Pap1 is controlled by the functional interaction between the positive regulator Pad1 and negative regulator Crm1. Both proteins are essential for cell viability and for the maintenance of chromosome structure. Pad1 is also responsible for resistance to staurosporine, and other drugs such as cycloheximide and caffeine.
Immunoblotting (dilution: 1/300 - 1/1000)
Optimal dilutions/concentrations should be determined by the end user.
Fractions from sucrose gradient centrifugation of wild type S. pombe cells containing integrated Cut8-3HA were immunoblotted using antibodies Nuc2, Pad1 and HA (ref.2).
Cut8 protein forms a broad peak around 4-15S (middle panel), distinct from the peak of 20S cyclosome (top panel) and 26S proteasome (bottom panel). Nuc2 and Pad1 are the subunits of cyclosome and proteasome, respectively.
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.