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T4 Gene 32 Protein (Single-stranded DNA Binding Protein, SSB)

Background

T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity (1, 2). It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.

Application

1. Promoting DNA replication and recombination by stabilizing single-stranded DNA (1)
2. Increase specificity and yields of long PCR products (3)

BAM_02040EX_1
Fig.1 SDS-PAGE of T4 SSB protein

BAM_02040EX_2
Fig.2 Binding activity to single-stranded DNA
0.02 ug/ul of M13mp18ssDNA was incubated with 0(lane0), 0.025 (lane1), 0.05 (lane2), and 0.1 (lane3) ug/ul of SSB at 37°C for 30 min and then 10 ul aliquot was subjected to electrophoresis in agarose.

Product List

Product Name Cat# Quantity Price

T4 gene 32 protein (Single-stranded DNA binding protein, SSB)

BAM-02-040-EX 200UG

¥ 10,000
$ 134
€ 100

T4 gene 32 protein (Single-stranded DNA binding protein, SSB)

BAM-02-040-5EX 5*200UG

¥ 34,500
$ 460
€ 345

References

1) Alberts BM & Frey L (1970) “T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA”. Nature 227:1313-1318 PMID:5455134


2) Bittner M et al (1979) “Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities” J Biol Chem 254: 9565-9572 PMID:226522


3) Schwarz K et al (1990) ”Improved yields of long PCR products using gene 32 protein” Nucleic Acids Res 18:1079 PMID:2107527

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.