Nucleobindin 2 (NUCB2), also known as NEFA or Nesfatin precursor, is a ubiquitously expressed EF-hand Ca2+ binding protein that is implicated in various physiological processes. Nucleobindin 2 interacts with the postmitotic growth suppressor necdin in neurons. Both necdin and nucleobindin 2 are expressed in differentiated neurons and skeketal muscles and these proteins are likely to be involved in the regulation of survival and death of postmitotic cells by controlling Ca2+ homeostasis.
1. Western blotting (1/3,000-1/1,000).
3. Immuno-cytochemistry (dilution: 1/1,000)
4. Immuno-histochemistry (dilution: 1/2,000)
5. Immuno-electron microscopy
6. Immuno-affinity chromatography
This antibody was produced and used in ref.3.
1. Barnikol-Watanabe S et al (1994) “Human protein NEFA, a novel DNA binding /EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein.” Biol Chem Hoppe-Seyler 375: 497-512 PMID: 7811391
2. Kroll KA et al (1999) “Heterologous overexpression of human NEFA and studies on the two EF-hand calcium-binding sites.” Biochem Biophys Res Commun 260:1-8 PMID: 10381334
3. Taniguchi N et al (2000) “The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm.” J Biol Chem 275: 31674-31681 PMID: 10915798
Anti Nucleobindin 2/ NEFA / Nesfatin precursor
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.