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Reagents for Genetic Engineerring

Pfu DNA Polymerase(-dNTPs), Economy

Background

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa, same as that of the natural Pfu DNA polymerase.

■ Pfu DNA polymerase is thermostabe and has low error rates.
■ It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
■ Pfu DNA polymerase-generated PCR fragments are blunt-ended.

Application

1) cloning
2) DNA expression
3) site-directed mutagenesis

General composition of PCR reaction mixture (total 50ml)
Pfu DNA polymerase (2.5 units/ml) 0.5 ml
10 x Reaction Buffer (Pfu) 5 ml
2.5mM (each) dNTPs 4ml
Template <500ng
Primer 1 0.2〜1.0mM (final conc.)
Primer 2 0.2〜1.0mM (final conc.)
Sterile distilled water up to 50ml

< SDS-PAGE of Taq DNA polymerase >

< Amplification of λ DNA >

PCR condition

98°C 10 sec
55°C 30 sec
72°C 10 min
} 30 cycles

(2 min in the case of 2 kb DNA)

Product List

Product Name Cat# Quantity Price

Pfu DNA polymerase (-dNTPs), Economy, with reaction buffer

BAM-02-031-EX 200UNIT

¥ 5,000
$ 67
€ 50

Pfu DNA polymerase (-dNTPs), Economy, with reaction buffer

BAM-02-031-5EX 5*200UNIT

¥ 20,000
$ 267
€ 200

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.