p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites, Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable, which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI. As to the kinase of phosphorylation of Ser46, involvement of DYRK2 is implicated.
This product is the purified IgG fraction obtained from serum free culture of mouse hybridoma (clone #36) which produces monoclonal antibody that specifically recognizes p53 protein with phospholyrated Ser46.
Data Link UniProtKB/Swiss-Prot P04637 (P53_HUMAN)
1) Western blotting (x1,000-2,000 dilution)
Other applications have not been tested.
Identification of Ser46-phosphorylated p53 protein by Western blotting.
Samples : Crude cell extracts of MOLT-4 untreated (left lanes) and treated with adriamycin for 24 h (right lanes).
The left panel is the result with our product and the right panel is the one obtained with the product of our competitor.
The lower panel is the whole p53 protein identified by omnipotent anti-p53 antibody.
Immunohistochemistry of stomach cancer.
Formalin/PFA-fixed paraffin-embedded section
1. Bode AM & Dong Z “Post-translational modification of p53 in tumorigenesis” Nature Rev Cancer 4: 793-805 (2004) PMID: 15510160
2. Oda K et al “p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53” Cell 102: 849-862 (2000) PMID: 11030628
3. Taira N et al “DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage” Mol. Cell 25:725-738 (2007) PMID: 17349958
To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.